Gastro-Protective Mechanisms of Aqueous Leaf Extract of Telfairia ocidentalis in Ethanol Induced - Gastric Ulceration in Spraque Dawley Rats
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Abstract
In terms of both morbidity and mortality gastric ulcer is a major health challenge and can induce upper gastrointestinal bleeding if not treated. This study evaluated the effects of aqueous leaf extract of Telfairia occidentalis on ethanol induced gastric ulcer in male spraque dawley rats. Twenty five adult male rats of comparable weight were divided into 4 groups of 5 rats each. Group A served as normal control, group B served as the test control and was ulcer induced with single oral administration of 5ml/kg body weight 75% absolute ethanol, group C received (500mg/kg) of T occidentalis, while group D was ulcer induced with single oral administration of 5ml 75% ethanol solution after pre-treatment with (500mg/kg) of T occidentalis. Another set of rats were distributed into various groups of 5 rats each and administered N-nitro-Larginine methyl ester 70 mg/kg, 1H[1,2,4] oxadiazolo [4,3-a]quinoxaline-1-one 10 mg/kg, glibenclamide, N-ethylmaleimide 5 mg/kg, and verapamil 5 mg/kg respectively. This was followed by admiration of T occidentalis extract and 5mL/kg body weight 75% absolute ethanol for gastric ulcer induction. Comparatively, the results of the study suggest that pre-treatment with T. occidentalis increased gastric wall mucus content, superoxide Dismutase, catalase, and prostaglandin but reduced gastric ulceration, oxidative stress, IL-8, and has apparent protective effect on gastric macroscopic architecture. Judging by the findings of this study, T occidentalis is highly gastroprotective, and possibly involving NP-SH, KATP, and Ca2+ channel to mediate its gastroprotective mechanism against ethanol induced gastric ulcer.
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