Proximate, Phytochemicals, and Mineral Analysis of Cola acuminata Aqueous Leaf Extract
Main Article Content
Abstract
Medicinal plants have been the focus of recent natural product research due to their safety and availability. Cola acuminata (CA) has been used in managing anemia, diarrhoea, cough, and dysentery. In this study, phytochemical composition, nutritional content, and In vitro antioxidant capacity of aqueous extract of CA leaves were ascertained. Fresh leaves of CA obtained from a farm in Benin City, Edo State, were air-dried and ground into powder. Exactly 500g of the powdered leaves was weighed and macerated in distilled water for 72 hours in an air-tight container with continuous stirring. This was filtered and concentrated with a rotary evaporator at 30ºC. Proximate analysis, phytochemical screening, and mineral analysis were carried out in accordance with standard methods. Results revealed nutritional content as crude fibre (31.7%), carbohydrate (31.24%), crude protein (4.7%), crude lipid (5.9%), moisture (16.22%), and ash (10.24%), phytochemical content includes cardiac glycosides, saponins, terpenoids, tannins and reducing sugars. Mineral analysis indicates high concentrations of magnesium (22.34 ± 0.34mg/100g) potassium (33.47 ± 1.34mg/100g), phosphorous (60.6 8± 1.26mg/100g) and Sodium (26.66 ± 0.51mg/100g) and low concentrations of calcium (8.63± 0.11mg/100g), zinc (1.63 ± 0.02mg/100g) and iron (4.50 ± 0.03mg/100g). The results show that CA leaves are a viable reservoir of natural products that are beneficial for the maintenance of health and drug design.
Metrics
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
References
Ekalu A, Habila JD. Phytochemistry, pharmacology and medicinal uses of Cola (Malvaceae) family: A review. Med. Chem. Res. 2020; 29:2089-105.
Veronique G, Greenwood K. Review taxonomic research and scientists study. J Bot Res. 2016;12(9):107-211.
Ichetaonye SI, Ajekwene KK, Ugo UK, Oguzie CK, Opara FA. A review of the characteristics and prospective applications of Cola acuminata (cola nut) dye extract on textile materials. Discov Mater. 2024;4(32). doi.org/10.1007/s43939-024-00095-5.
Okoli BJ, Abdullahi K, Myana O, Iwu G. Caffeine content of three Nigerian cola. J Emerg Trends Eng Appl Sci. 2012;3(5):830-3.
Onaolapo OJ, Onaolapo AY. Caffeinated beverages, behavior, and brain structure. In: Caffeinated and cocoa based beverages. Woodhead Publishing; 2019. p.163-207.
Umenwanne CL, Ogugofor MO, Njoku OU. Ethyl acetate fraction of Cola hispida leaf protects against doxorubicin-induced myocardial injury in male albino rats. Future J Pharm Sci. 2021;7:1-7.
Odebunmi E, Oluwaniyi O, Awoloa G. Proximate and nutritional composition of kola nut. Afr J Biotechnol. 2009;8(14):308-10.
Odugbemi T. Outlines and pictures of medicinal plants from Nigeria. Trop Agric. 2006;12(7):231-4.
Victoria OO, Stephen SO, Ruth OO, Rufina ASY. Antidiabetic and antioxidative potentials of aqueous extract of Cola acuminata leaves in alloxan-induced diabetes mellitus in male albino rats. Adv Anim Vet Sci. 2023;11(2):305-9.
AOAC International. Official methods of analysis of AOAC International. 17th ed. Gaithersburg (MD): AOAC International; 2000.
AOAC International. Official methods of analysis of AOAC International. 18th ed. Gaithersburg (MD): AOAC International; 2010.
Enabulele SA, Ehiagbonare JE. Preliminary qualitative phytochemical screening. Int J Phys Sci. 2011;5(6):753-62.
Pothiraj C, Balaji P, Shanthi R, Gobinath M, Babu RS, Munirah AAD, et al. Evaluating antimicrobial activities of Acanthus ilicifolius L. and Heliotropium curassavicum L. against bacterial pathogens: an in-vitro study. J Infect Public Health. 2021;14(12):1927-1934.
Owoeye TF, Akinlabu KD, Ajani OO. Proximate composition, phytochemical screening and mineral content studies of leaves extract of Adenanthera pavonine. Arab J Basic Appl Sci. 2023;30(1):317-28.
Olaniyi MB, Lawal IO, Olaniyi AA. Proximate, phytochemical screening and mineral analysis of Crescentia cujete L. leaves. J Med Plants Econ Dev. 2018;2(1):a28.
Dewole EA, Dewumi DFA, Alabi JYT, Adegoke A. Proximate and phytochemical analysis of Cola nitida and Cola acuminata. Pak J Biol Sci. 2013;16:1593-1596.
Ozoffor CH, Onyemelukwe NF, Ogbuagu E. Proximate analysis and pathological studies of extracts of Cola acuminata and Cola nitida in some visceral organs (kidney and liver) of albino rats in Southern Nigeria. Int J Curr Microbiol Appl Sci. 2024;13(4):15-25.
Brosnan J. Inter-organ amino acid transport and its regulation. J Nutr. 2006;133:2068-72.
Mettler S, Mitchell N, Tipton KD. Protein intake for body weight management in healthy adults. Nutrients. 2023;15(1):147.
Gorissen SH, Witard OC. Characterising the muscle anabolic potential of dairy, meat and plant-based protein sources in older adults. Proc Nutr Soc. 2018;77(1):20–31.
Dah-Nouvlessounon D, Adjanohoun A, Sina H, Noumavo PA, Diarrasouba N, Parkouda C, et al. Nutritional and anti-nutrient composition of three kola nuts (Cola nitida, Cola acuminata and Garcinia kola) produced in Benin. Food Nutr Sci. 2015;6:1395-407.
Adesuyi AO, Elumm IK, Adaramola FB, Nwokocha AGM. Nutritional and phytochemical screening of Garcinia kola. Adv J Food Sci Tech. 2012;4(1):9-14.
Pereira MA, O'Reilly E, Augustsson K, Fraser GE, Goldbourt U, Heitmann BL, et al. Dietary fiber and risk of coronary heart disease: a pooled analysis of cohort studies. Arch Intern Med. 2004;164(4):370-6.
Atanda OO, Olutayo A, Mokwunye FC, Oyebanji AO, Adegunwa MO. The quality of Nigerian kola nuts. Afr J Food Sci. 2011;5: 904-909.
Jo U, Park K. Carbohydrate Intake and Risk of Cardiovascular Disease: A Systematic Review and Meta-Analysis of Prospective Studies. Nutrients. 2023;15(7):1740.
Evuen UF, Kpoma ED. Phytochemical and Nutritional Constituents of Leaf Extracts of Two Edible Medicinal Plants in Nigeria: A Comparative Appraisal. J Complement Altern Med Res. 2023;23(1):9-21.
Bray GA, Kim KK, Wilding JPH. Obesity: a chronic relapsing progressive disease process. Lancet Diabetes Endocrinol. 2017;5(3):161–70. doi:10.1016/S2213-8587(16)30201-3
Durand D, Hubert A, Nafan D, Haziz S, Pacome AN, Farid B, et al. Antimicrobial, antioxidant, cytotoxic activities and phytochemical assessment of Cola acuminata used in Benin. Int J Pharmacol Biosci. 2015;7:102-9.
Zubair M, Niaz K, Iqbal J, Ali Z, Ali Q, Asif M. Ash content and mineral composition of medicinal plants: an overview. J Pharm Anal. 2021;11(1):30-5.
Ajai AI, Ochigbo SS, Jacob JO, Ndamitso MM, Abubakar U. Proximate and mineral composition of different species of kola nuts. Eur J Appl Eng Sci Res. 2012;1(3):44-7.
Mgbemena NM, Akoh OU, Obodo GA, Nwakwue K. Determination of the phytochemicals, minerals, proximate and antibacterial constituents of the leaf, stem, root and seed of Ficus capensis (Bush Fig). J Chem Soc Niger. 2022;47(1).
Isyaka MS, Al-Adam KB, Umar A, Adedoyin B, Mofio BM, Bemgba BN, Abdu Z, Jibril S, Rufai Y, Anyam JV, Igoli JO. Phytochemistry and Bioactivity of Nigerian Croton lobatus Stem Bark. Trop J Phytochem Pharm. Sci. 2024; 4(2): 79 – 84
Otoide JE, Olanipekun MK. Phytochemical study of underutilized leaves of Cola acuminata and Cola nitida. Am Res J Biosci. 2018;4(1):1-7.
Kanoma I, Muhammad I, Abdullahi S, Shehu K, Maishanu HM, Isah AD. Qualitative and Quantitative Phytochemical Screening of Cola Nuts (Cola nitida and Cola acuminata). J Biol Agric Healthcare. 2014;4(5):89-97.
Omwirhiren EM, James SA, Abass AO. The phytochemical properties and antimicrobial potentials of aqueous and methanolic seed extract of Cola nitida and Cola acuminata grown in South West, Nigeria. Saudi J Med Pharm Sci. 2016;2(12):354-63.
Madhu M, Sailaja V, Satyadev TN, Satyanarayana MV. Quantitative phytochemical analysis of selected medicinal plant species by using various organic solvents. J Pharmacogn Phytochem. 2016;5(2):25-9.
Oghogho UI, Ekugum E, Ogbeide OK, Idagan M, Uadia JO, Falodun A. Phytochemical Assessment, Anti-inflammatory and Antimalarial Activities of Beta vulgaris (Chenopodiaceae) Root Extract: Trop. J. Phytochem. Pharm. Sci. 2022;1(1):3-8.
Eleazu CO, Eleazu KC, Awa E, Chukwuma SC. Comparative study of the phytochemical composition of the leaves of five Nigerian medicinal plants. E3 J Biotechnol Pharm Res. 2012;3(2):42-6.
Navarrete P, Pizzi A, Pasch H, Rode K. Characterization of two maritime pine tannins as wood adhesives. J Adhes Sci Technol. 2013;27(22):2462-2479.
Sura Maan S, Nurhanan MY, Muhammad HJ, Yusof K. Cardiotonic effects of cardiac glycosides from plants of Apocynaceae family. Chula Med J. 2020;64(4):449-56
Soetan KO, Olaiya CO, Oyewole OE. The importance of mineral elements for humans, domestic animals and plants: a review. Afr J Food Sci. 2010;4(5):200-22.
Scherer R, Godoy HT. Antioxidant activity index (IAA) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chem. 2009;112:654-8.
Idris J, Shirai Y, Ando Y, Ali AAM, Othman MR, Ibrahim I, et al. Production of biochar with high mineral content from oil palm biomass. Malays J Anal Sci. 2014;18(3):700-4.
Idris S, Yisa J, Itodo J. Proximate and Mineral Composition of Abelmoschus esculentus. Int J Trop Agric Food Syst. 2009;3(2).
Okiki PA, Osibote IA, Balogun O, Oyinloye BE, Idris O, Adelegan O, et al. Evaluation of proximate, minerals, vitamins and phytochemical composition of Moringa oleifera Lam. cultivated in Ado Ekiti, Nigeria. Adv Biol Res. 2015;9(6):436-43.
Eze CN, Igboeli CN. Assessment of the Nutritional and mineral Composition of Pterocarpus Mildbreadii (Oha) Leaves. Int J Appl Sci Res. 2022;3(3).
Masitlha EP, Seifu E, Teketay D. Nutritional composition and mineral profiles of leaves of Moringa oleifera provenances grown in Garborne, Botswana. Food Prod Process Nutr. 2024;6(3).