Antimicrobial and Antioxidant Xanthone Derivative from Piliostigma thonningii leaf
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Abstract
Antimicrobial resistance and oxidative stress-related pathologies have guided the scientific community into the pursuit of natural product based research. The study aim to isolate and characterize antimicrobial and antioxidant compound(s) from the leaves of Piliostigma thonningii. Bioactivity-guided fractionation was carried out with the structure of the isolated compound elucidated using 1H and 13C NMR as well as liquid chromatography coupled mass spectrometry (LC-MS). The isolated compound was screened against standard bacteria strains of Staphylococcus aureus and Pseudomonas aeruginosa as well as fungal strains of Candida albicans and Aspergillus niger using agar well diffusion method. The inhibitory zone diameter (IZD) were determined and compared with reference antimicrobial ciprofloxacin and miconazole. The free radical scavenging activity in comparison with the ascorbic acid standard was performed using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay. The compound (8-hydroxyl-2,4,5-trimethoxyxanthone) displayed a lower antimicrobial and antioxidant activities in comparison to the positive standard employed. However, despite lower effect of the compound, this work opens the perspective to use this molecule as 'leads' for the design of novel and selective drug candidates of pharmaceutical relevance.
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References
Abdulrahman MD, Ali AM, Fatihah H, Khandaker MM, Mat N. Traditional medicinal knowledge of Malays in Terengganu, Peninsular Malaysia. Malay Nat J. 2018; 70: 349 -364
Abdulrahman MD. A systematic review on the biological evaluation of Calotropis procera (Aiton) Dryand. Future J. Pharm Sci. 2023; 9: 16
Dogara AM, Hamad SW, Hama HA, Bradosty SW, Kayfi S, Al-Rawi SS, Lema AA. Biological evaluation of Garcinia kola Heckel. Adv Pharmacol Pharm Sci. 2022; Article ID 3837965: 1 -15.
Abdulrahman MD, Zakariya AM, Hama HA, Hamad SW, Al-Rawi SS, Bradosty SW, Ibrahim AH. Ethnopharmacology, biological evaluation, and chemical composition of Ziziphus spina-christi (L.) Desf.: A Review. Adv Pharmacol Pharm Sci. 2022; Article ID 4495688: 1 - 36.
Festus BCO, Damian CO, Chukwunonso NA, Daniel LA, Thaddeus HG, Patience OO, Peter P. A new antioxidant and antimicrobial compound isolated from Alchornea cordifolia leaves. Afr J. Pharm Res and Dev. 2015; 7(2): 87 - 94
Resch A, Wilke M, Fink C. The cost of resistance: incremental cost of methicillin-resistant Staphylococcus aureus (MRSA) in German hospitals. Eur J. Health Econ. 2009; 10(3): 287 - 297
Soobrattee MA, Bahorun T, Aruoma OI. Chemopreventive actions of poly-phenolic compounds in cancer. Biofactors. 2006; 27: 19 - 35
Jones DP. Radical-free biology of oxidative stress. Am J. Physiol Cell Physiol. 2008; 295: C849 - 868
Kelly FT. Use of antioxidants in the prevention and treatment of disease. J. Int Fed Clin Chem. 1998; 10: 21- 23
Sasaki YF, Kawaguchi S, Kamaya A, Ohshita M, Kabasawa K, Iwama K, Taniguchi K, Tsuda S, The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutat Res/Genet Toxicol Environ Mutagen. 2002; 519: 103 - 109.
Vongtau HO, Abbah J, Chindo BA, Mosugu O, Salawu AO, Kwanashie HO, Gamaniel KS. Central inhibitory effects of the methanol extract of Neorautanenia mitis root in rats and mice. J. Pharm Biol. 2005; 43: 113 - 120.
Oluyemi KA, Okwuonu UC, Baxter DG, Oyesola TO. Toxic effects of methanolic extract of Aspilia Africana leaf on the estrous cycle and uterine tissues of wister rats. Int J. Morphol. 2007; 25: 609 - 614.
Igoli JO, Tor-Anyiin TA, Usman SS, Oluma HOA, Igoli NP. Folk medicines of the lower Benue valley of Nigeria. In V. K. Singh, J. N. Govil, S. Hashmi, and G. Singh, eds. Recent Progress in Medicinal Plants, Vol. 7. Ethnomed. Pharmacog. II. Sci. Tech. Pub. USA. 2002; 327 - 338 p.
Igoli JO, Ogaji OG, Tor-Anyiin TA, Igoli NP. Traditional medicine practice amongst the Igede people of Nigeria: Part II. Afr J. TradCompl and Altn Med. 2005; 2(2): 134 - 152.
Togola A, Diallo D, Dembélé S, Barsett H, Paulsen BS. Ethnopharmacological survey of different uses of seven medicinal plants from Mali (West Africa) in the regions Doila, Kolokani and Siby. J. Ethnobiol Ethnomed. 2005; 1: 7.
Ibewuike JC, Ogungbamila FO, Ogundaini AO, Okeke IN, Bohlin L. Anti-inflammatory and antibacterial activities of C-methylflavonols from Piliostigma thonningii. Phytother Res.1997; 11(4): 281 – 284.
Ogundaini A. 1999. Antimicrobial agents from some Nigerian plants. Nig J. Nat Prod Med.1999; 3: 26 - 27.
Fakae BB, Cambell AM, Barrett J, Scott IM, Teesdale-Spittle PH, Liebau E, Brophy PM. Inhibition of gluthathione S-transferases (GSTs) from parasitic nematodes by extracts from traditional Nigerian medicinal plants. Phytother Res. 2000; 14(8): 630 - 634.
Ukwueze SE, Osadebe PO, Okoye FBC. A new antibacterial benzoquinone glycoside from Psidium guajava (Linn). Nat Prod Res. 2015; 29: 1 - 7.
Jain A, Soni M, Deb L, Jain A, Rout S, Gupta V, Krishna K. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of Momordica dioica leaves, J. Ethnopharmacol. 2008; 115(1): 61 - 66.
Alaribe CS, Shode F, Herbert ABC, Ayoola G, Sunday A, Singh N, Iwuanyanwu S. (2011). Antimicrobial activities of hexane extract and Decussatin from stem bark extract of Ficus congensis. Int J. Mol Sci. 2011; 12: 2750 – 2756.
Peres V, Nagem TJ, Oliveria FF. Tetraoxygenated naturally occurring Xanthones. J. Phytochem. 2000; 55: 683 - 710.
Freitas VLS, da Silva MDMCR. Influence of hydroxyl functional group on the structure and stability of xanthone: A computational approach. Mols. 2018; 23: 2962.
Jumina J, Siswanta D, Zulkarnain K, Triono S, Priatmoko P, Yuanita E, Fatmasari N, Nursalim I. Development of C-arylcalix[4]resorcinarenes and C-arylcalix [4] pyrogallolarenes as antioxidant and UV-B protector. Indones J. Chem.2019; 19: 273 – 284