GC-MS Analysis of Five Commonly Used Herbal Formulations Sold in Anambra State, Southeast, Nigeria
DOI:
https://doi.org/10.26538/tjpps/v3i2.7Keywords:
Anticancer, Antimicrobial, Antiplasmodial, Antioxidant, Bioactive constituents, Herbal formulationsAbstract
Plant extracts have been known to possess multiple bioactive compounds with therapeutic uses. Gas chromatography-mass spectrometry (GC-MS) was used in the current study to characterize the bioactive constituents of five (5) commercially available herbal formulations. The National Institute of Standard Technology Database was used to analyze the mass spectrum of the five commonly available herbal formulations sold in Anambra State, Southeast, Nigeria. The GC-MS analysis showed the presence of thirty-five (35) bioactive compounds. The identified compounds were listed in the following order; Compound name, peak area (%), molecular weight, molecular formula, and biological activities. The bioactive compounds present include; Quinolone, Oleic acid, Hexadecanoic acid, Phenolic, Dodecanoic acid, and, phthalic acid which are believed to possess antiplasmodial, antimicrobial, antiviral, anticancer, antioxidant, and antifungal activities. This study confirmed that the five herbal formulations have rich bioactive compounds that could benefit mankind, although some of the synthetic compounds isolated are of major health concern.
References
Uraku AJ. Determination of chemical compositions of cymbopogon citratus leaves by Gas Chromatography-Mass spectrometry (GC-MS) method. Res. J. Phytochem. 2015; 9:175-187.
Obafemi TO, Afolabi CA, Mary TO, Amos O, Kayode CK, John AF. High performance liquid chromatography (HPLC) finger printing, mineral composition and in vitro antioxidant activity of methanol leaf extract of Synsepalum dulcificum (sapotaceae). App. Pharm. Sci. 2017; 7:110-118.
Uraku AJ, Okaka ANC, Ibiam UA, Agbafor KN, Obasi NA. Antiplasmodial activity of ethanolic leaf extracts of spilanthes uliginosa, Ocimum basilicum (sweet basicl), Hyptis spicigera and cymbopogon citrates on mice exposed to Plasmodium berghei Nk 65. J. Biochem. Res. Rev. 2015a; 6:28-36. https://doi.org/10.9734/IJBCRR/2015/9806
Uraku AJ, Offor CE, Itumoh EJ, Ukpabi CE, Aja PM. Gas chromatography-mass spectrometry (Gc-Ms) analysis of essential oil from Hyptis Spicigera leaves. Am. J. Boil. Chem. 2012b; 3:45-56.
Ighodaro OM, Ujomu TS, Asejeje FO, Adeosun AM, Subair SO. Toxicity and gas chromatography-mass spectrometry analyses of a polyherbal formulation commonly used in Ibadan metropolis, Nigeria. Toxicol. Rep. 2020; 7:1393–1401.
World Health Organization. Regulatory situation of herbal medicine A worldwide review. Geneva. 1998 [Accessed 2023 March 17]. https://apps.who.int/iris/handle/10665/63801.
Olabisi OT, Clement AA, Tolulope OM, Amos O, Kayode K, Olakunle AB, Akinwunmi AO, John FA, Aline BA, Linde AM. High performance liquid chromatography (HPLC) fingerprinting mineral composition and in vitro antioxidant activity of Methanol leaf extract of Synsepatum dulcificum (Sapotaceae). J. App. Pharm. Sci. 2017; 7:110-118. DOI: 10.7324/JAPS.2017.71117
Nwobodo DC, Eze PM, Okezie UM, Okafoanyali JO, Okoye FBC, Esimone CO. Bioactive compounds characterization and antimicrobial potentials of crude extract of Curvularia lunata, a fungal endophyte from Elaeis guineensis. Trop. J. Nat. Prod. Res. 2022; 6:395-402. DOI:10.26538/tjnpr/v6i3.16 .
Özçelik B, Kartal M, Orhan I. Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharm. Bio. 2011; 49:396–402.
Jelena SM, Zorica ZS, Mihailo SR, Jovana BV, Bojan KZ, Petar D, Ana MD. Chemical characterization, in vitro biological activity of essential
oils and extracts of three Eryngium L. species and molecular docking of selected major compounds. J. Food Sci. Technol. 2018; 55:2910–2925. https://doi.org/10.1007/s13197-018-3209-8.
Andrii M, Dennis EK, Roman M. 4(1H)-Pyridone and 4(1H)-Quinolone Derivatives as Antimalarials with Erythrocytic, Exoerythrocytic, and Transmission Blocking Activities. Curr Top Med Chem. 2014; 14:1693–1705.
Falah HOA. The Forgotten Role of Methenamine to Prevent Recurrent Urinary Tract Infection: Urgency for Reuse 100 Years After Discovery. Pharm. Biomed. Res. 2020; 6:247-250, http://dx.doi.org/10.18502/pbr.v6i4.5110.
Min-Gi K, Hoi-Seon L. 1,2-Benzendiol Isolated from Persimmon Roots and Its Structural Analogues Show Antimicrobial Activities against Food-borne Bacteria. J. Korean Soc. Appl. Biol. Chem. 2014; 57:429−433.
Ashwani K, Surender S, Sandeep J, Parvin K. Synthesis, antimicrobial evaluation, QSAR and in Silico ADMET studies of decanoic acid derivatives. Acta. Pol. Pharm. Dru. Res. 2011; 68:191-204. PMID: 21485292.
Ghazala BM, Shameel M, Chaudhary MI, Shahzad S, Leghari SM. Phytochemistry and bioactivity of two microalgae (Volvocophyta) from Sindh. Intern. J. Bio. Biotech. 2004; 1:343-350.
Sandhya S, Joyeeta T, Debabrat B. Chemical and Biological Properties of Lauric Acid: A Review. Intern. J. Ad. Res. 2016; 4:1123-1128.
Shereen AS, Mona MK, Rabab AM. Evaluation of the Antifungal Activity of Bacillus amyloliquefaciens and B. velezensis and Characterization of the Bioactive Secondary Metabolites Produced against Plant Pathogenic Fungi. Bio. 2022; 11:1390. https://doi.org/10.3390/biology11101390.
Duke JA. Handbook of Biologically Active Phytochemicals and Their Activities. (B. Raton & A. Ann, Eds.), Tokyo. 1992. p 183.
Zaha AE, Rajashri RN, Ashok KS, Sanaa KB. Fatty Acids Analysis, Antioxidant and Biological Activity of Fixed Oil of Annona muricata L. Seeds. J. Chem. 2016; Article ID 6948098: 1-6. http://dx.doi.org/10.1155/2016/6948098.
Özşen BÖ, Atlı Ö, Kıran İ. Biotransformation of oleic acid and antimicrobial and anticancer activities of its biotransformatıon extracts. Bulgarian Chem. Comm. 2019; 51:200-205, http://dx.doi.org/10.34049/bcc.51.2.4831.
Femi-Adepoju AG, Fatoba PO, Adepoju AO, Oluyori AP. Phytochemical analysis, antimicrobial activity and identification of phytoconstituents in Gleichenia pectinata (Willd.) C. Presl. Intern. J. Biomed. Adv. Res. 2018; 9:400-406.
Belakhdar G, Benjouad A, Abdennebi EH. Determination of some bioactive chemical constituents from Thesium humile Vahl. J. Mater. Environ. Sci. 2015; 6:2778-2783.
Osuntokun OT, Cristina GM. Bio isolation, chemical purification, identification, antimicrobial and synergistic efficacy of extracted essential oils from stem bark extract of Spondias mombin(Linn). Int J Mol Biol Open Access. 20019; 4:135‒143.
Ranganathan D. Phytochemical analysis of Caralluma nilagiriana using GC – MS. J. Pharm. Phytochem. 2014; 3:155-159.
Habib MR, Karim MR. Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhy-drosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower. Mycobiol. 2009; 37:31-36.
Seow L, Beh H, Ibrahim P, Sadikun A, Asmawi ZA. Antimicrobial activity of Gynura segetum’s leaf extracts and its active fractions. Asso. Hum. Med. 2012; 2:e20. DOI:10.5667/tang.2012.0010.
Kumar S, Bawa S, Gupta H. Biological Activities of Quinoline Derivatives. Mini. Rev. Med. Chem. 2009; 9:1648-1654. DOI: 10.2174/138955709791012247.
Phaechamud T, Mahadlek J, Charoenteeraboon J, Choopun S. Characterization and Antimicrobial Activity of NMethyl2pyrrolidoneloaded Ethylene OxidePropylene Oxide Block Copolymer Thermosensitive Gel. Indian J. Pharm. Sci. 2012; 74:498-504. DOI: 10.4103/0250-474X.110574
Beulah GG, Soris PT, Mohan VR. GC-MS Determination of Bioactive Compounds of Dendrophthoe Falcata (L.F) Ettingsh: An Epiphytic Plant. Intern. J. Hea Sci. Res. 2018; 8:261-269.
Rajabi L, Courreges C, Montoya J, Aguilera RJ, Primm TP. Acetophenones with selective antimycobacterial activity. Let. Appl. micro. 2005; 40:212-217. https://doi.org/10.1111/j.1472-765X.2005.01657.x.
Ingole AS, Kadam MP, Dalu AP, Kute SM, Mange PR, Theng VD, Lahane OR, Nikas AP, Kawal YV, Nagrik SU, Patil PA. A Review of the Pharmacological Characteristics of Vanillic Acid. J. Dru. Del. Therap. 2021; 11:200-204 DOI: http://dx.doi.org/10.22270/jddt.v11i2-s.4823.
Chukwuma IF, Nworah FN, Apeh VO, Omeje KO, Nweze EJ, Asogwa CD, Ezeorba TTP. Phytochemical Characterization, Functional Nutrition, and Anti-Diabetic Potentials of Leptadenia hastata (pers) Decne Leaves: In Silico and in vitro Studies. Bioinform. Bio. Ins. 2022; 16:1–17.
Hassan AS. The Antibacterial Activity of Dimethyl Sulfoxide (DMSO) with and without of Some Ligand Complexes of the Transitional Metal Ions of Ethyl Coumarin against Bacteria Isolate from Burn and Wound Infection. J. Na. Sci. Res. 2014; 4:2224-3186.
Jafari E, jarah-Najafabadi NT, Jahanian-Najafabadi A, Poorirani S, Hassanzadeh F, Sadeghian-Riz S. Synthesis and evaluation of antimicrobial activity of cyclic imides derived from phthalic and succinic anhydrides. Res. Pharm. Sci. 2017; 12:526-534. http://dx.doi.org/10.4103/1735-5362.217433
Sanni DM, Omotoyinbo OV. GC-MS Analysis of Pteleopsis suberosa Stem Bark Methanol-Chloroform Extract. J. Pl. Sci. 2016; 4:37-40.
Neeraj N, Vasudeva S, Sharma S. Chemical composition of Fagopyrum esceulentum Moench seed through GC-MS. Intern. J. Pharm. Sci. Res. 2019; 10:2392–2396.
Shobi TM, Viswanathan MBG. Antibacterial activity of di-butyl phthalate isolated from Begonia malabarica. J. Appl. Biotech. Bioeng. 2018; 5:97–100.
Ahsan T, Chen J, Zhao X, Irfan M, Wu1 Y. Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf. AMB Expr. 2017; 7(1):54. DOI: 10.1186/s13568-017-0351-z
Mishra PM, Sree A. Antibacterial Activity and GCMS Analysis of the Extract of Leaves of Finlaysonia obovata (A Mangrove Plant). Asian J. Plant Sci. 2007; 6:168-172. DOI: 10.3923/ajps.2007.168.172
Uma B, Parvathavarthini R. Antibacterial Effect of Hexane Extract of Sea Urchin, Temnopleurus alexandri (Bell,1884). Inter. J. Pharm. Tech. Res. 2010; 2:1677-1680.
Ayoola GA, Coker HAB, Adesogun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC. (2008) Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop. J. Pharm. Res. 2008; 7:1019-24.
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