Polyphenol Profile and Antioxidant Properties of Various Solvent Fractions of <I>Phyllanthus amarus</I>

Authors

  • Gabriel O. Ibobo Department of Chemical Sciences (Biochemistry Option), Novena University, Ogume, Delta State, Nigeria
  • Joel Okpoghono Department of Biochemistry, Delta State University of Science and Technology, Ozoro, Delta State, Nigeria
  • Innocent Onyesom Department of Medical Biochemistry, Delta State University, Abraka, Delta State, Nigeria

DOI:

https://doi.org/10.26538/tjpps/v3i3.6

Keywords:

solvent, Polyphenol, Phyllanthus amarus, Methanol, Diethyl ether, Antioxidant

Abstract

The prevention of various diseases and the promotion of health are the major functions of natural antioxidants. In the current study, the polyphenols, phytochemicals and antioxidants contents of various fractions of P. amarus obtained through fractionation were examined. Liquid-liquid partition separation was used in separating the P. amarus crude extract into various fractions (methanol, diethyl ether, n-hexane, n-butanol, and aqueous fractions). The amount of polyphenols were assessed using high performance liquid chromatography (HPLC). Phytochemicals and antioxidants assay were performed with standard methods. The phytochemical screening of P. amarus crude extract and different fractions revealed the presence of saponins (4.00 ± 0.50 mg/dL), tannins (7.50± 1.20 mg/dL), alkaloids (6.40 ± 0.05 mg/dL), steroids (1.55 ± 0.15 mg/dL), and flavonoids (6.00 ± 1.02 mg/dL). There was a significant improvement in the DPPH scavenging activity, flavonoids, total phenol, FRAP, ABTS, TAC, and ascorbate oxidase when comparing the crude extract to various fractions. Nine polyphenols such as o-salicyclic acid, caffeic acid, p-anisic acid, protocatechuic acid, gallic acid, ferulic acid, vanillic acid, p-coumaric acid and sinapinic acid were targeted in crude extract and different fractions. The total polyphenol content of crude extract and various fractions were as follows; crude extract > methanol fraction > diethyl ether fraction > n-hexane fraction > n-butanol fraction > aqueous fraction. Overall, diethyl ether and methanol were found to be the best solvents for the extraction of antioxidant compounds from P. amarus leaves.

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References

Onyesom I, Mordi J, Opajobi AO, Esume CO. Hepatoprotective Potentials Of Hibiscus Rosasinensis Petal anthocyanin Extracts Against Carbon tetrachloride-Induced Acute Liver Damage in Wistar Rats. Sudan Med J Sci. 2008; 3(1):33-36.

Antonioni A, Fantini C, Dimauro I, Caporossi D. Redox homeostasis in sport: do athletes really need antioxidant support? Res Sports Med. 2019; 27:147–165.

Ekakitie LI, Okpoghono J, Orororo CO, Ekakitie AO.. Ameliorative prowess of bee honey in the tissues of rats administered aluminium nitrate. Sci Afr. 2021; 12: e00782.

Okpoghono J, Isoje FE, Igbuku AU, Ekayoda O, Omoike OG, Adonor OT Igue UB, Okom SU, Ovowa OF, Stephen-Onojedje OQ, Ejueyitsi OE, Seigha AA. Natural Polyphenols: A Protective Approach to Reduce Colorectal Cancer. Heliyon 2024a; 10 (11): e32390, DOI:https://doi.org/10.1016/j.heliyon.2024.e32390.

Okpoghono J, George BO, Achuba FI. Assessment of antioxidant indices after incorporating crude oil contaminated catfish and African nutmeg (Monodora myrstica) extracts into rat diet. J. Appl. Sci. Environ. Manag. 2018a; 22 (2) 197 – 202.

Okpoghono J. Preservative approaches, culinary and medicinal prospective mechanisms of herbs and spices: A Compiled Update. 2024; Available at SSRN: https://ssrn.com/abstract=4671664 or http://dx.doi.org/10.2139/ssrn.4671664

Onyesom I, Uzuegbu UE, Adu FO, Ayeta GE. Phytosuppression of Plasmodium berghei multiplication by Nauclea latifolia improves the indicies of pancreatic beta cell function and anemia in experimental mice. Nig Q J Hosp Med. 2015a; 25:142-144.

Okonta CI, Okpoghono J, George BO, Joseph O, Obiejogo J. Changes in lipid profile and antioxidant levels of rats fed cycas formulated diet and clove (Syzygium aromaticum) extract. Trop J Nat Prod Res. 2021; (5): 959-962.

Okpoghono J, Ukperegbulem JK, Igue UB. Anti-lipidemic and protein restoration potential of Monodora myristica (Gaertn.) in rats fed with cassava containing crude oil. Foods Raw Mater. 2024b;12(2):249–255.

Omoike GO, George BO, Ugwu EO, Okpoghono J. (). Changes in the antioxidant properties of Ocimum africanum leaves aqueous extract from the south eastern region of Nigeria after in vitro gastrointestinal digestion. Int J Food Prop. 2022; 25(1): 2000-2008.

Okpoghono J, Ukperegbulem JK, Onyesom I. Neuroprotective aftermath of Monodora myristica and Glycyrrhiza glabra against cassava diet containing vacuum gas oil induced brain injury in Wistar rats. Toxicol Reports 2024c;12 244-252.

Uzuegbu UE, Opajobi OA, Utalor JE, Elu CO, Onyesom I. Cytotoxicity and antiplasmodial activity of alkaloid extracts prepared from eight African medicinal plants used in Nigeria. Thai J Pharm Sci. 2020; 44(4):237-244

Okpoghono J, Achuba FI, George BO. Differential Solvent extraction on the antioxidant properties, and antioxidant compounds of Monodora myristica. Sokoto J. Med Lab Sci. 2018c; (1): 88-100.

Zhou XL, Yang YM, Guan JX, Yan Y. Prediction of asphalt aging time history based on infrared spectrum. Sino-Foreign Highw.2020; 40: 218–223.

Okom SU, Ojugbeli ET, Okpoghono J, Onyesom I. Safety and antimalarial therapeutic index of alkaloid-rich extract of Phyllanthus amarus Schumach. & Thonn. in mice. Heliyon. 2023; 9 (12): e23078.

Onyesom I, Onumaechi IF, Ehiwario J, Dagana R. Antiplasmodial activity of Phyllanthus amarus preserves renal function in Plasmodium berghei infected mice. European J Med Plants 2015b; 5(1):109-116.

Adjene JO, Nwose EU. Histological effects of chronic administration of Phyllanthus amarus on the kidney of adult Wistar rat. North Am J Med Sci. 2010; 2:193-195.

Ashwlayan, V. D. and Singh, R. (2011). Reversal effect of Phyllanthus emblica (Euphorbiaceae) Rasayana on memory deficits in mice. Int J Appl Pharm. 3: 10–15.

Amurun SO, Okpoghono J, Anunuso CI Health risk assessment of polychlorinated biphenyls exposure from inland rivers sediments in Warri-South, Warri, Delta State. Ovidius Univ Ann Chem. 2024;35(1):1-7

Okpoghono J, Osioma E, Metie SO, Igue UB, Isoje EF, Okom SU. Xylopiaa ethiopica HPLC-DAD polyphenol profiling and antioxidant status from South-South region of Nigeria. Ovidius Uni Ann Chem.2023a; 34(2):104 – 111.

Okpoghono J, Jacdonmi Igue UB, Precious I, Isoje EF, Okom SU, Stephen-Onojedje, O.Q. (). Comparative study of polyphenol profile after stimulated post-intestinal digestion of raw and dried ginger leaves. Ann Sci Technol. 2023b;8(2) 22-29.

Sofowora A. Medicinal Plants and Traditional Medicine in Africa, Spectrum Books Ltd., Ibadan, Nigeria, 1993; Pp. 289-290.

Harborne JB. Phytochemical Methods, London, Chapman and Hall, Ltd. 1973; 49-188

Ursini F, Maiorino M, Morazzoni P, Roveri A, Pifferi G. A novel antioxidant (1dB 1031) affecting molecular mechanisms of cellular. Free Radic Biol Med. 1994;16:547-53.

Ebrahimzadeh MA, Hosseinimehr SJ, Hamidinia A, Jafari M. Antioxidant and free radical scavenging activity of Feijoa sallowiana fruits peel and leaves. Pharmacology 2008; 1:7-14.

Dewanto X, Wu K, Adom, K, Liu RH. Thermal processingenhances the nutritional value of tomatoes by increasingtotal antioxidant activity, J Agric Food Chem. 2002; 50: 3010–3014.

Benzie IFF, Devaki M. The ferric reducing/antioxidant power (FRAP) assay for non-enzymatic antioxidant capacity: concepts, procedures, limitations and applications. Meas Antioxid Act Cap. 2017; 77–106.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cationdecolorization assay. Free Radic Biol Med. 1999; 26 (9–10): 1231-1237.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitationof antioxidant capacity through the formation of a phospho-molybdenum complex: specific application to the determination of vitamin E. Anal Biochem.1999; 269: 337–341.

Vines HM, Oberbacher MF. Response of oxidation and phosphorylation in Citrus mitochondria to arsenate. Nature 1965; 206: 319-320.

Seal T. Quantitative HPLC analysis of phenolic acids, flavonoids and ascorbic acid in four different solvent extracts of two wild edible leaves in India. J Appl Pharm Sci.. 2016; 6 (02): 157-166.

El-mahmood MA, Doughari JH, Chanji FJ. In vitro antibacterial activities of crude extract of Naucloa lantifolia and Daniella oliveri. Sci Res Essay 2008; 3(3): 102-105.

Ahmed W. Monitoring antioxidant and antityrosinase activity of clove aromatic flower buds. J Med Plants Stud. 2016; 4(2): 163-169.

Abiola MA, Sarah OO, Osasenaga MI, Okikiola HD. Omotayo MO. Phytochemical, minerals and free radical scavenging profiles of Phoenix dactilyfera L. seed extract. J Taibah Univ Med Sci. 2016;11(1):1-6.

Okpoghono J, Omoriare UJ, Igue UB, Seigha AA. Evaluation of liver and kidney function markers of mature albino male rats fed with maize-plantain pudding delicacy cooked in metallic plates and edible plant leaves. J Appl Sci Environ Manag. 2023 27 (4), 759-766.

Ejueyitsi EO, George BO, Anigboro AA, Okpoghono J. Antioxidant and polyphenol indices of popularly consumed indigenous soups in Delta State, Nigeria. Food Human.2023; 1:297-307.

Boo, Y.C. Can Plant Phenolic Compounds Protect the Skin from Airborne Particulate Matter? Antioxidants. 2019;8:379-382

ZhouY, Jiang Z, Lu H, Xu Z, Tong R, Shi J, Jia G. Recent Advances of Natural Polyphenols Activators for Keap1-Nrf2 Signaling Pathway. Chem Biodivers. 2019; 16:e1900400.

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Published

2024-07-03

How to Cite

Ibobo, G. O., Okpoghono, J., & Onyesom, I. (2024). Polyphenol Profile and Antioxidant Properties of Various Solvent Fractions of <I>Phyllanthus amarus</I>. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(3), 246–253. https://doi.org/10.26538/tjpps/v3i3.6