Phytochemistry and Bioactivity of Nigerian <I>Croton lobatus</I> Stem Bark
DOI:
https://doi.org/10.26538/tjpps/v4i2.8Keywords:
Bioassay, Croton lobatus, Euphorbiaceae, Phytochemical Investigation, Medicinal PlantsAbstract
Croton lobatus L. (Euphorbiaceae) is an important medicinal plant commonly used in traditional medicine. In this study, the C. lobatus plant was subjected to detailed phytochemical investigation with the view to isolate and characterize its dichloromethane and methanol stem bark extracts. Using silica gel column chromatography, lupeol, lupenone, and octylferulate, were isolated and subsequently characterized by nuclear magnetic resonance (NMR) spectroscopy, and gas chromatography–mass spectroscopy (GC-MS). The evaluation of the antioxidant and cytotoxicity potentials of the crude extracts and isolated compounds showed remarkable antioxidant activity at variable concentrations (0.5 mg/mL, 1 mg/mL, and 1.5 mg/mL), highlighting the therapeutic prospects of C. lobatus L. Furthermore, the stem bark extracts exhibited high toxicity, as evidenced by an LC50 value of 58.012 μg/mL observed during the brine shrimp toxicity assay. The study findings not only add to the understanding of the phytochemical composition of C. lobatus L. but also highlights the plant's potential as a source of bioactive chemicals with st-rong cytotoxic and antioxidant effects. Lastly, the findings will facilitate more pharmacological research and therapeutic applications by providing insightful information aboutthe plant's potential for medicinal use
References
Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: Pharmacological and toxicological considerations. J Ethnopharmacol. 2014;155(2):857–924.
Odukoya OA, Sofidiya MO, Samuel AT, Ajose I, Onalo M, Shuaib B. Documentation of Wound Healing Plants in Lagos-Nigeria : Inhibition of Lipid Peroxidation as In-vivo Prognostic Biomarkers of Activity. Ann Biol Res. 2012;3(4):1683–1689.
Gbolade AA. Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria. J Ethnopharmacol. 2009;121(1):135–139.
Lagnika L, Weniger B, Senecheau C, Sanni A. Antiprotozoal activities of compounds isolated from Croton lobatus l. African J Infect Dis. 2010;3(1):1–5.
Attioua B, Weniger B, Chabert P. Antiplasmodial Activity of Constituents Isolated from Croton lobatus. Pharm Biol. 2007;45(4):263–266.
Félix TZ, Attioua BK, Harisolo R, Boti JB, Adiko VA, Tonzibo FZ, Djakoure LA. Isolation and identification of alkaloids from Croton lobatus. Res Attioua, R Harisolo, JB Boti, VA Adiko, FZ Tonzibo, LA DjakoureInt J Pharm Sci Rev Res, 2012•researchgate.net. 2012;13(2).
Stuart KL, Woo-Ming RB. Vomifoliol in Croton and Palicourea species. Phytochemistry. 1975;14(2):594–595.
Isyaka MS, Akintayo AH, Umar A, Adedoyin BA, Nyakuma BB, Zakari A, Mofio BM, Rufai Y, Igoli JO. Lupeol and crotocorylifuran from Nigerian Croton gratissimus: Unlocking potent bioactive compounds. J Essent Oil Plant Compos. 2024;2(3):163–170.
Pellegrini N, Del Rio D, Colombi B, Bianchi M, Brighenti F. Application of the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation assay to a flow injection system for the evaluation of antioxidant activity of some pure compounds and beverages. J Agric Food Chem. 2003;51(1):260–264.
Mailafiya MM, Pateh UU, Hassan HS, Sule MI, Bila AH, Musa TL, Atinga V, Achika JI. Isolation of lupeol from the stem bark of Leptadenia hastata (Pers.) Decne. J Appl Sci Environ Manag. 2020;24(10):1835–1838.
Adedoyin BA, Adeniran OI, Muhammed AB, Dangoggo SM, Nahar L, Sharples GP, Sarker SD. Isolation and characterization of propitious bioactive compounds from Cassia singueana L. Adv Med Plant Res. 2020;8(4):89–100.
Isyaka SM, Mas-Claret E, Langat MK, Hodges T, Selway B, Mbala BM, Mvingu BK, Mulholland DA. Cytotoxic diterpenoids from the leaves and stem bark of Croton haumanianus (Euphorbiaceae). Phytochemistry. 2020;178.
Ferdinand C, Isyaka MS, Adelakun TA, Ibekwe NN, Igoli J, Abdullahi AM. Isolation of sitosterol and stigmasterol from the roots of croton penduliflorus. Fudma J Sci. 2023;7(6):52–56.
Chan SW, Mahmoud VL, Wang X, Teoh ML, Loh KM, Ng CH, Wong WF, Looi CY. Chemical profiling and cytotoxicity screening of agarwood essential oil (Aquilaria sinensis) in brine shrimp nauplii and cancer cell lines. PLoS One. 2024;19(11):e0310770–e0310770.
Puapairoj P, Naengchomnong W, Kijjoa A, Pinto MM, Pedro M, Nascimento MSJ, Silva AMS, Herz W. Cytotoxic activity of lupane-type triterpenes from Glochidion sphaerogynum and Glochidion eriocarpum two of which induce apoptosis. Planta Med. 2005;71(3):208–213.
Isyaka MS. The chemistry of African Croton species. [Surrey]: University of Surrey; 2020.
Ahamed AA, Ibrahim Adam YS, Hussien AM, Hassan ME. Effect of Heat Treatment on Antioxidant and Antimicrobial Activity of Croton gratissimus and Xylopiaaethiopica Spices. J Agric Environ Vet Sci. 2020 1;4(1).
Dominique Chodaton-Zinsou M, Mahoudo Assogba F, Yayi-Ladékan E, Gbaguidi F, Moudachirou M, Djimon Gbénou J. Phytochemical Composition, Biological Activities of <i>Croton lobatus L.</i> Leaves, Hydrolysis Effect on Activities and Chemical Composition. Am J Appl Chem. 2020;8(1):13.
Deore SL, Khadabadi SS, Baviskar BA, Khadabadi SS, Khangenbam RA, Koli US, Daga NP, Gadbail PA, Jain PA. In vitro antioxidant activity and phenolic content of Croton caudatum. Int J ChemTech Res. 2009;1(2):174–176.
Qaisar MN, Chaudary BA, Uzair M, Hussain SN, Qaisar MN, Chaudary BA, Uzair M, Hussain SN. Evaluation of Antioxidant and Cytotoxic Capacity of Croton bonplandianum. Baill. Am J Plant Sci. 2013;4(9):1709–1712.
Dossou SSK, Xu F, You J, Zhou R, Li D, Wang L. Widely targeted metabolome profiling of different colored sesame (Sesamum indicum L.) seeds provides new insight into their antioxidant activities. Food Res Int. 2022;151.
Antioxidant Profiling, Phytochemical Investigation and Pharmacognostic Evaluation of Nephrolepis biserrata (SW.) Schott (Nephrolepidaceae). Trop J Phytochem Pharm Sci. 2024;3(2).
Haematological, Renal and Gastric Effects of Ethanol Leaf Extract of Bauhinia variegate Linn. Trop J Phytochem Pharm Sci. 2024;3(2).
Mustanir, Ginting B, Murniana, Khaliza N, Fitria A. Toxicity test with brine shrimp lethality test (BSLT) method of annonacea family. Vol. 3082, AIP Conference Proceedings. AIP Publishing; 2024. p. 40040.
Vanhaecke P, Persoone G, Claus C, Sorgeloos P. Proposal for a short-term toxicity test with Artemia nauplii. Ecotoxicol Environ Saf. 1981;5(3):382–387.
McLaughlin JL, Rogers LL, Anderson JE. The Use of Biological Assays to Evaluate Botanicals. Drug Inf J. 1998;32(2):513–524.
Solis PN, Wright CW, Anderson MM, Gupta MP, Phillipson JD. A microwell cytotoxicity assay using Artemia salina (brine shrimp). Planta Med. 1993;59(3):250–252.
Elumba ZS, Teves FG, Malaluan RM. DNA-binding activity and in vivo cytotoxicity of extracts. African J Microbiol Res. 2013;7(3):202–210.
Malinowska M, Sikora E, Ogonowski J. Production of triterpenoids with cell and tissue cultures. Acta Biochim Pol. 2013;60(4):731–735.
Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med. 1982;45(1):31–34.
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