In vivo Evaluation of the Anti-Diarrhoeal Therapeutic Effects of Costus afer Methanol Extract
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Abstract
Herbal medicines are traditionally used for prophylaxis and therapeutic purposes. This research work explored the anti-diarrhoeal properties of methanol extract of Costus afer stem (MECAS) on diarrhea induced by castor oil in male albino rats. The experimental animals were randomly separated into six groups, each consisting of six (6) albino rats. Group 1 served as the standard control; group 2 was administered normal saline while group 3 was given loperamide (2 mg/kg); groups 4, 5, and 6 were treated with varying doses of the MECA (200, 400, and 600 mg/kg b.w). The effects of the MECAS on standard defecation showed that the groups treated with 200 mg/kg b.w MECAS and loperamide reduced defecation compared to those that received 400 and 600 mg/kg b.w MECAS with 2.33 ± 1.53 and 3.67 ±1.36 fecal droppings respectively. The result indicated a significant (p<0.05) dose-dependent increase in the percentage inhibition of propulsion (35.16%) and enteropooling inhibition in the group administered 200 mg/kg b.w MECAS compared to the groups that received 400 and 600 mg/kg b.w MECAS respectively. This research demonstrated that the Costus afer exhibits anti-diarrhoeal effects at lower doses for the treatment of diarrhea patients and scouring animals.
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