Effects of Acute Administration of Aqueous Extract From Sepals of Hibiscus Sabdariffa on Renal Excretion of Water In Albino Rats

Abstract

The effect of acute administration of extract of Hibiscus sabdariffa on renal excretion of water has been investigated. Sprague Dawley rats of either sex weighing between 150 - 250 g were anesthetized with a mixture of 25 % urethane and 1 s% alphachloralose, and their femoral vein was cannulated intravenous administration of drugs, the bladder was annulated for timely collection of urine. Acute intravenous administration of the extract of Hibiscus sabdariffa (5 mg/kg/hr) increased urine output (p<0.05), glomerular filtration rate (p<0.001), and renal excretion of Na+and K+. On the other hand, acute oral administration of extract of Hibiscus sabdariffa (5mg/kg/hr) had little effect on urine output, glomerular filtration rate, and renal excretion of Na+ and k+. Indomethacin, a prostaglandin synthesis inhibitor, had no alterations on the impact of Hibiscussabdariffaon renal excretion of water. L- NG-Nitro argenine methyl ester (L-NAME) did not alter the ability of Hibiscus sabdariffa to promote renal excretion of water. This suggests that the action of Hibiscus sabdariffa does not involve the synthesis of prostaglandins, nor is it mediated through the stimulation of endothelium-dependent relaxation factors.