HPLC and FT-IR Analyses of Nigerian Alligator Pepper (<i>Aframomum melegueta</i>) Methanol Seed Extract

Authors

  • Folasade M. Makinde Food Science and Technology Programme, College of Agriculture, Engineering and Science, Bowen University, Iwo, Osun State, Nigeria.
  • Etido F. Udo Medical Laboratory Sciences Programme, College of Health Sciences, Bowen University, Iwo, Osun State, Nigeria.
  • Dolapo D. Olugbile Medical Laboratory Sciences Programme, College of Health Sciences, Bowen University, Iwo, Osun State, Nigeria.
  • Oduola O. Abiola Medical Laboratory Sciences Programme, College of Health Sciences, Bowen University, Iwo, Osun State, Nigeria.

DOI:

https://doi.org/10.26538/tjpps/v3i3.3

Keywords:

HPLC, FT-IR, phytochemical, Aframomum melegueta

Abstract

Traditional knowledge had been established to successfully preserve and restore biodiversity across the continent since it accommodates indigenous herbalism. Traditional medicine had been adopted in Nigeria prior to the colonialism era. The study examined the phytochemical constituents in methanolic seed extract of alligator pepper (Aframomum melegueta) grown in Nigeria by Fourier Transform Infrared Spectrophotometer (FT-IR) and High Performance Liquid Chromatography (HPLC) analyses. Aframomum melegueta seeds were extracted with 70% methanol at room temperature for 48 h. The functional groups in aqueous methanolic seed extract were determined using FT-IR spectroscopic method while the bioactive compounds were determined using HPLC. The FT-IR result established the presence of N-H, C=N, C=C, C-O and O-H functional groups in the methanolic extract. The HPLC analysis of the methanolic extract identified ten compounds. The major components were farnesyl (37.11%), lycopene (34.15%), dihydrocapsiacin (9.55%), capsaicin (8.40%) and delphenidin (7.91%). Other compounds present in the extract at low levels were phytoene (0.74%), β-lonone (0.57%), limonene (0.56%), 6-methoxymellen (0.56%) and trans-beta-ocimene (0.45%). In essence, this study established the ethnobotanical use of Aframomum melegueta as functional food ingredient.

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Published

2024-07-03

How to Cite

Makinde, F. M., Udo, E. F., Olugbile, D. D., & Abiola, O. O. (2024). HPLC and FT-IR Analyses of Nigerian Alligator Pepper (<i>Aframomum melegueta</i>) Methanol Seed Extract. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(3), 230–233. https://doi.org/10.26538/tjpps/v3i3.3