Tannase Production from Different Strains of <i>Rhodotorula species</i> using <i>Chrysophyllum albidum</i> Seed Shell Flour as Substrate
DOI:
https://doi.org/10.26538/tjpps/v3i4.6Keywords:
tablet properties, dissolution, disintegration, starch, Persea AmericanaAbstract
Tannin is one of the major components of plants material. Tannase is an enzyme that breakdown tannin which is commonly implicated as an anti-nutritional factor in food and feed products. This study is aimed to determine the potential of Chrysophyllum albidum seed shell flour as substrate for microbial production of tannase. Rhodotorula species was isolated, screened and utilized for tannase production. A novel substrate, Chrysophyllum albidum seed shell flour, was unveiled for tannase production by solid state fermentation in this study. Six Rhodotorula species were isolated and characterized as Rhodotorula graminis, Rhodotorula rubra, Rhodotorula mucilaginosa, Rhodotorula minuta, Rhodotorula toruloides and Rhodotorula glutinis from thirteen soil samples obtained from different locations in Abeokuta, Ogun State, Nigeria. Only Rhodotorula mucilaginosa CBS 316, Rhodotorula toruloides strain bca-286 and Rhodotorula glutanis CBS20 were best tannase producers after screening and identification using molecular tools. Tannase activities from medium formulated with C. albidum seed shell flour were 542.45 U, 513.39 U and 489.50 U for R. mucilaginosa CBS 316, R. toruloides strain bca-286 and R. glutanis CBS20 respectively. Tannase activities were 610.75 U, 508.40 U and 485.85 U for R. mucilaginosa CBS 316, R. toruloides strain bca-286 and R. glutanis CBS20 respectively using medium formulated with wheat bran and sugar cane bagasse. This study indicated the potential of Chrysophyllum albidum seed shell flour as a novel substrate for tannase from strains of Rhodotorula species.
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