Comparative Studies of Agro Wastes for Pectinase Production and Characterization by Strains of Aspergillus niger
Keywords:Pectinase, pineapple peel, corn pomace, orange peel, Aspergillus niger
Pectinase (EC 126.96.36.199) are often described as enzymes that breakdown pectin to give glucose and galaturonic acid. It is an inducible extracellular enzyme often produced by microorganisms. This investigation was done to compare three agro wastes (Orange peel, pineapple peel and corn pomace) for microbial pectinase production by solid state fermentation. Pectinase was precipitated with ammonium sulphate and characterized. Optimum incubation period of both strains of Aspergillus niger were 92 hrs with each agro wastes. Among the agro wastes, orange peel had the highest activity of 101.372±0.042 and 108.820±0.127 U for A. niger strain A1 and A2 respectively. Ammonium sulphate concentration of 80% indicates maximum specific activity for pectinase produced from both A. niger strains. Optimum pH and temperature of 5 and 50 ºC were observed for pectinase produced by both strains. Cu 2+ , Zn 2+ , Mg 2+ , Fe 2+ , Na 2+ , Ca 2+ and Mn 2+ moderately activate pectinase activity. K + tends to exert inhibitory effect on pectinase from both A. niger strains
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