Biochemical Studies, Antimicrobial Activity and Green Synthesis of Aqueous Extract (AE) of <I>Citrus aurantium</I> Leaves
DOI:
https://doi.org/10.26538/tjpps/v4i2.10Keywords:
Aqueous Extract, Antioxidant Potential, Antimicrobial Activity, Green Synthesis, Metallic NanoparticlesAbstract
The goal of this study was to examine the biochemical properties and green synthesis characterization of the aqueous extract (AE) of Citrus aurantium leaves. Exactly 20 grams of the air-dried leaves were ground into powder and mixed with 100 ml of distilled water and ethanol, which were separately shaken overnight and filtered to obtain aqueous and ethanol extracts that were then used for the analyses,using conventional analytical techniques of phytochemical analyses, in vitro antioxidant studies, green synthesis, and antimicrobial studies. The research findings indicated that the AE displayed a wide range of the phytochemical constituents under investigation when compared the ethanol extract. The total phenolic content concentration rose from 20 mg/mL to 100 mg/mL as well as in flavonoids concentration. The FTIR spectra of the C. aurantium showed different absorption peaks for the AE (3951, 3997) cmˉ¹,CuNP (3991, 3967) cmˉ¹, AgNP (3945, 3198) cm̉¹, and ZnNP (3950, 3694) cmˉ¹, respectively. The green synthesis of synthesized nanoparticles of the Citrus aurantium also showed different absorption peaks. Citrus aurantiuma queous extract and its silver, zinc, and copper nanoparticles exhibited a significant percentage of mycelia growth inhibition on the two fungi species (Trichophyton verrycosum and Epidermophyton floccosum) under investigation. These compounds demonstrated strong antibacterial activity by inhibiting the growth zones of four bacteria: Ralstonia solanacearum, E. coli, Pseudomonas glycinea, and Staphylococcus aureus. From the study, C. aurantium was observed to possess medicinal properties to justify its use in ethno-medicinal treatment of diseases that can be used for drug development.
References
García-Salas P, Gómez-Caravaca AM, Arráez-Román D, Segura-Carretero A, Guerra-Hernández E, García-Villanova B, Fernández-Gutiérrez A. Influence of technological processes on phenolic compounds, organic acids, furanic derivatives, and antioxidant activity of whole-lemon powder. Food Chem. 2013;141(2):869–878.
Khan H, Nabavi SM, Sureda A. Therapeutic potential of songorine, a diterpenoid alkaloid of the genus. Aconitum. Eur J Med Chem. 2017. In press.
Putnik P, Bursać Kovačević DR, Barba JA, Cravotto F, Binello A, Lorenzo JM, Shpigelman A. Innovative “green” and novel strategies for the extraction of bioactive added-value compounds from citrus wastes: A review. Molecules. 2017;22(5):680.
USDA-Foreign Agricultural Service (USDA-FAS). Citrus: World markets and trade. Washington (DC): Foreign Agricultural Service; 2020.
Mannucci C, Calapai F, Cardia L, Inferrera G, D’Arena G, Di Pietro M, Navarra M, Gangemi S, Ventura SE, Calapai G. Clinical pharmacology of C. aurantium and Citrus sinensis for the treatment of anxiety. Evid Based Complement Altern Med. 2018:3624094.
Nabavi SF, Khan H, D'Onofrio G. Apigenin as neuroprotective agent: of mice and men. Pharmacol Res. 2018; 128:359–365.
Mayers DL, Lerner SA, Ouellette M. Antimicrobial drug resistance: clinical and epidemiological aspects. Vol. 2. Dordrecht (Netherlands): Springer; 2009. p. 681–1347.
Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem. 2011;13(10):2638.
Sofowora A. Medicinal plant and traditional medicine in Africa. Ibadan (Nigeria): Spectrum Books Limited; 2006. 289 p.
Trease GE, Evans WC. Pharmacognsy. 11th ed. Brailliar Tiridel Can: Macmillan Publishers; 1989.
Harborne JB. A guide to modern techniques of plant analysis. 3rd ed. London (UK): Chapman and Hall; 1998. 285 p.
Talaiekhozani A, Amani AM. Enhancement of cigarette filter using MgO nanoparticles to reduce carbon monoxide, total hydrocarbons, carbon dioxide, and nitrogen oxides of cigarette. J Environ Chem Eng. 2019;7(1):102873.
Gyamfi MA, Yonamine M, Aniya Y. Free radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally induced liver injuries. Gen Pharmacol. 1999;32(6):661–667.
Jagetia GC, Baliga MS. The evaluation of nitric oxide scavenging activity of certain Indian medicinal plants in vitro: A preliminary study. J Med Food. 2004;7(3):343–348. doi:10.1089/1096620041938740.
Pulido R, Bravo L, Saura-Calixto F. Antioxidant activities of dietary phenols are determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem. 2000;48(8):3396–3402. doi:10.1021/jf9913458.
Minotti G, Aust SD. An investigation into the mechanism of citrate-Fe²⁺-dependent lipid peroxidation. Free Radic Biol Med. 1987;3(6):379–387.
Puntel RL, Nogueira CW, Rocha JBT. Krebs cycle intermediates modulate thiobarbituric acid reactive species (TBARS) production in rat brain in vitro. Neurochem Res. 2005;30(2):225–235.
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999; 299:152–178.
Roe JH, Kuether CA. The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid. J Biol Chem. 1943; 147:399–407.
Okwu DE. Phytochemicals and vitamin content of indigenous spices of South Eastern Nigeria. J Sustain Agric Environ. 2004;6(1):30–37.
Terry P, Giovannucci E, Michels KB, Bergkvist L, Hansen H, Holmberg L, Wolk A. Fruit, vegetables, dietary fiber, and risk of colorectal cancer. J Natl Cancer Inst. 2001;93(7):525–533.
Okwu DE. Investigation into the medicinal and nutritional potentials of Garcinia kola Heckel and Dennettia tripetala. G. Baker. PhD Thesis. Michael Okpara University of Agriculture, Umudike, Nigeria. 2003;20–31.
Burt SA. Essential oils: their antibacterial properties and potential applications in foods: a review. Int J Food Microbiol. 2004;94(3):223–253.
Kawaii S, Yasuhiko T, Eriko K, Kazunori K, Masamichi OY, Meisaku K, Chihiro I, Hiroshi F. Quantitative study of flavonoids in leaves of citrus plants. J Agric Food Chem. 2000;48(9):3865–3871.
Silalahi J. Hypocholesterolemic factors in foods: a review. Indon Food Nutr Prog. 2000; 7:26–35.
Okwu DE, Emenike IN. Evaluation of phytonutrients and vitamin content of citrus fruit. Int J Mol Med Adv Sci. 2006;2(1):1–6.
Yano M, Kawaii S, Tomono Y, Katase E, Ogawa K. Quantification of flavonoid constituents in citrus fruits. J Agric Food Chem. 1999;47(9):3565–3571.
Del-Rio A, Obdululio BG, Casfillo J, Main FG, Ortuno A. Uses and properties of citrus flavonoids. J Agric Food Chem. 1997;45(12):4505–4515.
Kouhbanani MAJ, Beheshtkhoo N, Taghizadeh S, Amani AM, Alimardani V. One-step green synthesis and characterization of iron oxide nanoparticles using aqueous leaf extract of Teucrium polium and their catalytic application in dye degradation. Adv Nat Sci: Nanosci Nanotechnol. 2019;10(1):015007.
Shahraki RR, Ebrahimi M, Seyyed Ebrahimi SA, Masoudpanah SM. Structural characterization and magnetic properties of superparamagnetic zinc ferrite nanoparticles synthesized by the coprecipitation method. J Magn Magn Mater. 2012;324(22):3762–3765.
Bock NA, Kocharyan A, Liu JV, Silva AC. Visualizing the entire cortical myelination pattern in marmosets with magnetic resonance imaging. J Neurosci Methods. 2009;185(1):15–22.
Zhang KL, Zhou J, Zhou H, Wu Y, Liu R, Wang LL, et al. Bioinspired “active” stealth magneto-nanomicelles for theranostics combining efficient MRI and enhanced drug delivery. ACS Appl Mater Interfaces. 2017;9(36):30502–30509.
Cheong SJ, Lee CM, Kim SL, Jeong HJ, Kim EM, Park EH, et al. Superparamagnetic iron oxide nanoparticles loaded chitosan-linoleic acid nanoparticles as an effective hepatocyte-targeted gene delivery system. Int J Pharm. 2009;372(1–2):169–176.
Mousavi SM, Hashemi SA, Ghasemi Y, Amani AM, Babapoor A, Arjmand O. Applications of graphene oxide in nanomedicines and nanocarriers for biomolecules: a review study. Drug Metab Rev. 2019;51(1):12–41.
Li M, Cushing SK, Wu N. Plasmon-enhanced optical sensors: a review. Analyst. 2015;140(2):386–406. doi:10.1039/c4an01079e.
Oladunmoye MK, Ayantola KJ, Agboola AA, Olowe BM, Adefemi OG. Antibacterial and FTIR spectral analysis of methanolic extract of Gliricidia sepium leaves. J Adv Microbiol. 2018;9(4):1–10. doi:10.9734/JAMB/2018/40580.
Gopal PV. Evaluation of anti-microbial activity of Citrus aurantium against some gram-positive and gram-negative bacterial strains. Pharmacia. 2012; 1:107–109.
Aibinu I, Adenipekun T, Adelowotan T, Ogunsanya T, Odugbemi T. Evaluation of the antimicrobial properties of different parts of Citrus aurantifolia (lime fruit) as used locally. Afr J Trad CAM. 2007;4(2):185–190.
Balamurugan S. In vitro antifungal activity of Citrus aurantifolia Linn plant extracts against phytopathogenic fungi Macrophomina phaseolina. Int Lett Nat Sci. 2014; 13:70–74. doi:10.18052/www.scipress.com/ILNS.13.70.
Makhlouf KE, Boungab K, Elouissi A, Daikh ZE. Antifungal activity of Citrus aurantium L. essential oil against crown rot of wheat caused by Fusarium graminearum. Pak J Phytopathol. 2022;34(2):201–211. doi:10.33866/phytopathol.034.02.0798.
Liu Y, Benohoud M, Yamdeu JHG, Gong YY, Orfila C. Green extraction of polyphenols from citrus peel by-products and their antifungal activity against Aspergillus flavus. Food Chem X. 2021; 12:100144. doi: 10.1016/j.fochx.2021.100144.
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