<i>In vitro</i> Evaluation Of The Hypoglycaemic, Anti-Inflammatory, And Antioxidant Activities Of <i>Tragia benthamii</i> Baker (EUPHORBIACEAE)
DOI:
https://doi.org/10.26538/tjpps/v3i9.4Keywords:
Antioxidant, Anti-inflammatory, Hypoglycemic, Tragia benthamiiAbstract
Tragia benthamii Baker (Euphorbiaceae) is a climbing herb with significant traditional uses in Africa, particularly in Western Africa. This study aimed to evaluate the invitro hypoglycemic, anti-inflammatory, and antioxidant effects of Tragia benthamii leaves. The alpha-amylase inhibition and membrane stabilization assays were employed to evaluate the invitro hypoglycemic and anti-inflammatory activities of the methanol extract, n-hexane, chloroform, and ethylacetate fractions. Additionally, the antioxidant activity of the methanol crude extract was investigated using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and ferric-reducing antioxidant power (FRAP) assays. The IC50 values obtained for the alpha-amylase inhibition were 63.52±0.069, 193.9±0.077, 913.19±0.044, and 3559.89±0.057 µg/mL for the methanol extract, n-hexane, chloroform, and ethylacetate fractions, respectively, while the reference drug sample, acarbose, exhibited an IC50 value of 455.30±0.063 µg/mL. IC50 values for the anti-inflammatory activity were 82.75±0.005, 75.06±0.02, 64.92±0.014, and 105.74±0.056 µg/mL for the methanol extract, n-hexane, chloroform, and ethylacetate fractions, respectively, and the reference drug, diclofenac, had an IC50 value of 53.26±0.002 µg/mL. Moreover, the IC50 values for the DPPH assay were 46.07±0.043, 53.78±0.008, and 7.43±0.008 µg/ml for the crude methanol extract, vitamin E, and vitamin C, respectively. The FRAP assay demonstrated that the leaf extract exhibited a total antioxidant activity of 185 µM Fe2+/g dry extract, whereas vitamin C (positive control) showed an activity of 405 µM Fe2+/g. Overall, this study indicates the potential hypoglycemic, anti-inflammatory, and antioxidant properties of T. benthamii leaf extracts.
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