Phytochemical and Antimicrobial Studies of Stored Dried Leaves of <i>Alchornea Cordifolia</i> (Shum &Thonn) Mull. Arg. (Euphorbiaceae)


  • Ayokunnumi F. Obajuluwa Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Kaduna State University, Kaduna, Nigeria
  • Comfort L. Dani Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria
  • Gbonjubola O. Adeshina Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria
  • Stephen K. Parom Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Kaduna State University, Kaduna, Nigeria
  • Mujidat Bello Department of Food and Industrial Biotechnology, National Biotechnology Research and Development Agency, Abuja, Nigeria



bactericidal concentration, inhibitory concentration, antimicrobial properties, Alchornea cordifolia


Background: Herbal medicines are promising remedies in the fight against antimicrobial resistance and the readily availability of target medicinal plants all year round is very essential. This study was aimed at assessing the antimicrobial properties of dried leaves of Alchornea cordifolia after being stored for five and a half years.Ethanol and hot water maceration, and soxhlet extraction of the stored dried leaves of Alchornea cordifolia were carried out followed by phytochemical screening. Antimicrobial test of the extracts against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans was carried out using agar cup plate and disc diffusion methods. Minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of the plant extracts against the test organisms were conducted.Phytochemical screening revealed the presence of tannins, saponins and flavonoids. Both the aqueous and ethanol extracts were active against all the tested organisms: S. aureus was the most susceptible followed by E. coli, Ps aeruginosa, and C. albicans. The MIC values of the extracts against C. albicans, and Ps. aeruginosa were 20 mg/ml, 10 mg/ml and 10 mg/ml for hot water macerated extract, ethanol macerated extract and ethanol Soxhlet extract. The extracts of leaves of A. cordifolia were found to be active against the microorganism tested even after the leaves were stored for five and a half years. The activities observed were similar to that of fresh A. cordifolia leaves


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How to Cite

Obajuluwa, A. F., Dani, C. L., Adeshina, G. O., Parom, S. K., & Bello, M. (2024). Phytochemical and Antimicrobial Studies of Stored Dried Leaves of <i>Alchornea Cordifolia</i> (Shum &Thonn) Mull. Arg. (Euphorbiaceae). Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 3(2), 196–200.