Molluscicidal Activity of Aqueous Methanol and Hexane Fractions of Tetrapleura tetraptera Stem Bark against Biomphalaria pfeifferi
DOI:
https://doi.org/10.26538/tjpps/v3i8.1Keywords:
Molluscicides, Mortality, Tetrapleura tetraptera, Dose-response, Phyto-constituentAbstract
Snail infections and parasitic diseases associated with snails such as schistosomiasis remain a problem in some endemic communities in Nigeria. Consequently, effective molluscicides are needed to control these parasites. However, frequent use of commercially available molluscicides may cause unnecessary environmental pollution. This necessitates the advocacy for natural alternatives to commercially available synthetic molluscicides. This study aimed to investigate the molluscicidal activity of aqueous methanol and hexane fractions of Tetrapleura tetraptera stembark against Biomphalaria pfeifferi snails. A total of two hundred and eighty (280) mature snails from Giri River, Gwagwalada Area Council of the Federal Capital Territory (FCT), Abuja, Nigeria, were subjected to immersion bioassay with methanol and hexane fractions of Tetrapleura tetraptera stembark. Dechlorinated water was used as negative control while CuSO4.5H2O (aq) was employed as positive control. Treatment concentration ranged from 0.39 mg/L to 12.5 mg/L and snail death was measured at 24, 48, and 72 hours post-exposure. The results revealed that the aqueous methanol fraction of Tetrapleura tetraptera stembark have high molluscicidal activity with LC90 of 2.184 ± 0.61 mg/L and LC50 of 0.647± 0.61 mg/: after 24 hours exposure time; while the hexane extract showed no significant toxicity towards the test organism. This observation may be attributed to the presence of tannins glycosides or carbohydrates prominent in the aqueous methanol fraction and were not present in the hexane fraction. Findings from this study provide considerable basis for further exploiting the aqueous methanol fraction of Tetrapleura tetraptera stembark and local indigenous plants for as potential molluscicidal agents.
References
Sachs-Barrable K, Conway J, Gershkovich P, Ibrahim F, Wasan KM. The use of the United States FDA programs as a strategy to advance the development of drug products for neglected tropical diseases. Drug Dev Ind Pharm. 2014; 40(11):1429-1434.
Adenowo AF, Oyinloye BE, Ogunyinka BI, Kappo AP. Impact of human schistosomiasis in sub-Saharan. Africa. Braz J Infect Dis. 2015; 19(2):196-205.
Gouveia MJ, Brindley PJ, Gärtner F, Costa JMC, da Vale N. Drug repurposing for schistosomiasis: Combinations of drugs or biomolecules. Pharmaceuticals (Basel). 2018; 11(1):15.
Noël H, Ruello M, Maccary A, Pelat C, Sommen C, Boissier J, Barré-Cardi H, Fillaux J, Termignon JL, Debruyne M. Large outbreak of urogenital schistosomiasis acquired in Southern Corsica France: Monitoring early signs of endemicization. Clin Microbiol Infect. 2018; 24(3):295-300.
Utzinger J, Raso G, Brooker S, De Savigny D, Tanner M, Ørnbjerg N, Singer B, N’goran E. Schistosomiasis and neglected tropical diseases: Towards integrated and sustainable control and a word of caution. Parasitol. 2009; 136(13):1859-1874.
Dawaki S, Al-Mekhlafi HM, Ithoi I, Ibrahim J, Abdulsalam AM, Ahmed A, Sady H, Nasr NA, Atroosh WM. The menace of schistosomiasis in Nigeria: Knowledge attitude and practices regarding schistosomiasis among rural communities in Kano state. PLoS One. 2015; 10(11):e0143667.
Dawaki S, Al-Mekhlafi HM, Ithoi I, Ibrahim J, Abdulsalam AM, Ahmed A, Sady H, Atroosh WM, Al-Areeqi MA, Elyana FN. Prevalence and risk factors of schistosomiasis among Hausa communities in Kano State Nigeria. Rev Inst Med Trop Sao Paulo. 2016; 58:54.
Chitsulo L, Engels D, Montresor A, Savioli L. The global status of schistosomiasis and its control. Acta Trop. 2000; 77(1):41-51.
Oyeyemi OT, de Jesus Jeremias W, Grenfell RFQ. Schistosomiasis in Nigeria: Gleaning from the past to improve current efforts towards control. One Health. 2020; 11:100183.
Ifeanyi CI, Matur BM, Ikeneche NF. Urinary schistosomiasis and concomitant bacteriuria in the Federal Capital Territory Abuja Nigeria. NY Sci. 2009; 2:1–8.
Bishop H. Menace of schistosomiasis: Its true neglected nature in Nigeria. MOJ Public Health. 2017; 6(5):421–426.
Anyanti J, Akuiyibo S, Onuoha O, Nwokolo E, AtagameK,Braid E. Addressing schistosomiasis in a community in Nigeria: A theoretical approach. Int J Trop Dis. 2021; 4(1):1-6.
McCullough F, Gayral P, Duncan J, Christie J. (1980) Molluscicides in schistosomiasis control. Bull World Health Organ. 1980; 58(5):681-689.
Bakry F and Hamdi S. Molluscicidal activity of latex aqueous solution of Euphorbia acetonitril and Euphorbia granulate against the intermediate hosts of schistosomiasis and fascioliasis. J U Arab Bio. 2007; 27:101–126.
Tantawy A, Mostafa B, Sharaf El-Din A. Molluscicidal activity of Synadenium grantii (Euphorbiaceae) against Biomphalaria alexandrina and Bulinus truncatus the intermediate host snails of schistosomiasis in Egypt and their infectivity with the parasite. J Sci. 2004; 14:183–196.
Li J and Xu H. Bioactive compounds from the bark of Eucalyptus exserta F Muell. Ind. Crops Prod. 2012; 40:302–306.
Marston A, Maillard M, Hostettmann K. Search for antifungal molluscicidal and larvicidal compounds from African medicinal plants. J Ethnopharmacol. 1993; 38(2-3):215-223.
Marston A and Hostettmann K. Review article number 6: Plant molluscicides. Phytochem. 1985; 24(4):639–652.
Parkhurst R, Mthupha B, Liang Y, Bruce J, Lambert J, Collier T, ApSimon J, Wolde-Yohannes L, Heath G, Jones W. The molluscicidal activity of Phytolacca dodecandra I Location of the activating esterase. Biochem Biophysic Res Cummun. 1989; 158:436–439.
Ojewole JA and Adewunmi CO. Anti-inflammatory and hypoglycaemic effects of Tetrapleura tetraptera (Taub) [fabaceae] fruit aqueous extract in rats. J Ethnopharmacol. 2004; 95(2-3):177-182.
Al-Thobaiti SA and Zeid IMA. Phytochemistry and pharmaceutical evaluation of Balanites aegyptiaca: an overview. J Exp Bio Agric Sci. 2018; 6(3):453 – 465.
Ojewole JA. Analgesic and anticonvulsant properties of Tetrapleura tetraptera (Taub)(Fabaceae) fruit aqueous extract in mice. Phytother Res. 2005; 19(12):1023-1029.
Aladesanmi AJ. Tetrapleura tetraptera: Molluscicidal activity and chemical constituents. Afr J Tradit Complement Altern Med. 2006; 4(1):23-36.
Adesina S, Ojewole J, Marquis V. Isolation and identification of an anticonvulsant agent from the fruit of Tetrapleura tetraptera (Aridan/Aidan). The Nig J Pharm. 1980; 11(6):260–262.
Ojewole J and Adesina S. Mechanism of the hypotensive effect of scopoletin isolated from the fruit of Tetrapleura tetraptera. Planta Med. 1983; 49(1):46-50.
Lewis WH. The Useful Plants of West Tropical Africa. Econ Bot 40, 176 [online]. 1986 [cited 2024 Aug. 15]. Available from: https://doi.org/10.1007/BF02859140
Enwere NJ. Foods of Plant Origin. University of Nigeria, Nsukka: Afro-Orbis Publications Limited; 1998. 194 p.
Adelaja B and Fasidi I. Survey and collection of indigenous spice germplasm for conservation and genetic improvement in Nigeria Plant Genetic Resources. Agric Food Sci. 2008; 153:67–71.
Banu KS and Cathrine L. General techniques involved in phytochemical analysis. Int J Adv Res Chem Sci. 2015; 2(4):25–32.
Afolayan M, Srivedavyasasri R, Asekun OT, Familoni OB, Orishadipe A, Zulfiqar F, Ibrahim MA, Ross SA. Phytochemical study of Piliostigma thonningii a medicinal plant grown in Nigeria. Med Chem Res. 2018; 27(10):2325–2330.
Yadav R and Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol. 2011; 3(12):10-14.
FAO. Molluscicides for the control of freshwater snails: A review of the current-status. FAO Fisheries and Aquaculture Technical Paper No 580. 2013; 1-220 p.
Ibeh C and Walmsley B. The role of impact assessment in achieving the sustainable development goals in Africa. Int Assoc Impact Assess. 2021; 1-15 p.
Michael ES, Yole D, Musila MF, Kutima H, Kareru P. Assessment of Molluscicidal, Cercericidal, and Miracicidal Activities of Crude Extracts of Azadirachta indica and Entada leptostachya. J Biol Agric Healthcare. 2013; 3(5):11-17.
Ayi I, Chandre F, Coelho P, El-Harawy A, Elemam M, Gachuhi K, Jian-Rong D, Kariuki C, Madsen H, Moné H. Guidelines for laboratory and field testing of molluscicides for control of schistosomiasis.WHO. 2019; 115-200 p.
Saidu U, Ibrahim MA, de Koning HP, McKerrow JH, Caffrey CR, Balogun EO. Human schistosomiasis in Nigeria: Present status diagnosis chemotherapy and herbal medicines. Parasitol Res. 2023; 122(12):2751-2772.
Koma OS, Olawumi OO, Godwin EU, Theophilus OA. Phytochemical screening in-vitro antimicrobial activity and antioxidant characteristics of Tetrapleura tetraptera extracts. Eur J Med Plants. 2016; 17(2):1-10.
Olusola A, Olajide OO, Afolayan M, Khan I. Preliminary phytochemical and antimicrobial screening of the leaf extract of Cassia singueana. Afr J Pure Appl Chem. 2011; 5(4):65-68.
Han X, Shen T, Lou H. Dietary polyphenols and their biological significance. Int J Mol Sci. 2007; 8(9):950–988.
Okwu DE. Phytochemicals and vitamin content of indigenous spices of South Eastern Nigeria. J Sustainab Agric Env. 2024; 6:30-34.
Sodipo O, Akinniyi JA, Ogunbameru J. Studies on certain characteristics of extracts of bark of Pausinystalia johimbe and Pausinystalia macroceras (K Schum) Pierre ex Beille. Glob J Pure Appl Sci. 2000; 6(1):83–88.
Epand RF, Savage PB, Epand RM. Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins). Biochim Biophys Acta. 2007; 1768(10):2500-2509.
Nobori T, Miura K, Wu DJ, Lois A, Takabayashi K, Carson DA. Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers. Nature. 1994; 368(6473):753–756.
Njoku OV and Obi C. Phytochemical constituents of some selected medicinal plants. Afr J Pure Appl Chem. 2009; 3(11):228–233.
Okwu D. Evaluation of chemical composition of medicinal plants belonging to Euphorbiaceae. Pak Vet J. 2001; 14: 60–162.
Okwu D and Okwu M. Chemical composition of Spondias mombin Linn plant parts. J Sustainab Agric Env. 2004; 6(2): 140-147.
Stray F. The Natural Guide to Medicinal Herbs and Plants. London: Tigers books Int; 1998. 12 p.
Joseph A, David O, Simon-Oke I. The efficacy of three indigenous plants (Tetraplura tetraptera Bridelia ferruguinea and Azadiractha indica) as plant derived molluscicides against freshwater snails. Int J Trop Dis. 2019; 2(1):2–6.
Oniya M, Adigun D, Afolabi O. Evaluation of the molluscicidal activity of oil extracts of Tetrapleura tetraptera and Xylopia aethiopica on Bulinus globosus (Mollusca; Planorbidae). Nig J Parasitol. 2013; 34(1):21–24.
Adesina S, IwalewaE, Johnny I. Tetrapleura tetraptera Taub- ethnopharmacology chemistry medicinal and nutritional values-a review. Br J Pharm Res. 2016; 12(3):1–22.
Joshi RK. A perspective on the phytopharmaceuticals responsible for the therapeutic applications. In: Pharmaceutical Sciences: Breakthroughs in Research and Practice. 2017; 229 – 262 p.
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