Phytochemical and Antioxidant Activity of the Ethanol Extracts of Songgolangit Plant (<i>Tridax procumbens</i> L.)
DOI:
https://doi.org/10.26538/tjpps/v3i5.4Keywords:
Gas Chromatography-Mass Spectrometry, antioxidant, phytochemistry, Tridax procumbensAbstract
The songgolangit plant is used by some Indonesians as a traditional wound medicine. Therefore, this study was conducted to determine the content of phytochemical compounds and antioxidant activity in ethanol extracts of songgolangit plants (Tridax procumbens L.) so that the use of this plant is more widespread and scientifically proven to contain compounds that can function as drugs. This research uses the GC-MS (Gas Chromatography-Mass Spectrometry) method because this method can separate compounds that are mixed together and can identify various compounds even in low concentrations. Antioxidant activity testing uses the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method, this method is often used because it is fast, simple, and does not require high costs for screening free radical capture activity. Extract testing via GC-MS showed that the leaf sample of the songgolangit plant had 15 compounds, the stem sample had 11 compounds, and the flower sample had 11 compounds. Only three compounds were found equally in both leaf, stem, and flower. These compounds were 1,2,5-oxadiazol-3-carboxamide, 4,4'-azobis-, 2,2'-dioxide with a function as an antifungal activity. The 4,6-dichloro-5,7-dinitro-2,1,3-benzothiadiazole compound has been no specific research on this compound. Meanwhile 5,8-epoxy-15-nor-labdane was a compound that had antidiabetic function. Songgolangit plants contain alkaloids, tannins, terpenoids, flavonoids, steroids, organic compounds, fatty acids, amines, naphthalene acids and catecholamines. Antioxidant activity analysis showed that songgolangit plants had an IC50 value of 81 µg/mL in leaf, 80 µg/mL in stem, and 81µg/mL in flower, and all had strong antioxidant activity category.
References
Beck S, Mathison H, Todorov T, Calderón-Juárez EA, Kopp OR. A review of medicinal uses and pharmacological activities of Tridax procumbens (L.). J. Plant Stud. 2018; 7(1): 19-35.
Pradeep M, Franklin G. Understanding the hypericin biosynthesis via reversible inhibition of dark gland development in Hypericum perforatum L. Industrial Crops and Products. 2022; 18(2): 114876.
Balfagón D, Terán F, de Oliveira TDR, Santa-Catarina C, Gómez-Cadenas A. Citrus rootstocks modify scion antioxidant system under drought and heat stress combination. Plant Cell Reports. 2021; 1-10.
Qaderi MM, Martel AB, Strugnell CA. Environmental Factors Regulate Plant Secondary Metabolites. Plants. 2023; 12(3): 447.
Aguirre GO, Taco MMM., Huchin VMM, Rangel HAL, Jiménez JAR, Vázquez AG, Dzib-Cauich DA, Pérez EVB, Canul AJC. Effect of Extraction Type on Bioactive Compounds and Antioxidant Activity of Moringa oleifera Lam. Leaves. Agriculture. 2022; 12(9): 1462-1462.
Gomes AF, Almeida MP, Leite MF, Schwaiger S, Stuppner H, Halabalaki M, Amaral JG, David JM. Seasonal variation in the chemical composition of two chemotypes of Lippia alba. Food Chem. 2019; 273: 86-193.
Li Y, Kong D, Fu Y, Sussman MR, Wu H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol. Biochem. 2020; 148: 80-89.
Awaliyah S, Widiyanto SNB, Maulani RR, Husyari UD, Syamsudin TS, Marwani E. Correlation of Microclimate of West Java on Caffeine and Chlorogenic acid in Coffea canephora var. robusta. J Biotech Sci, Tech, and Man. 2022; 4(1) :54-60.
Matsui K, Walker AR. Biosynthesis and regulation of flavonoids in buckwheat. Breed Sci. 2020; 70(1): 74-84.
Iheanacho CM, Akubuiro PC, Oseghale IO, Imieje VO, Erharuyi O, Ogbeide KO, Jideonwo AN, Falodun A. Evaluation of the Antioxidant Activity of the Stem Bark Extracts of Anacardium occidentale (Linn) Anacardiaceae: http://www.doi.org/10.26538/tjpps/v2i2.4. Trop J Phytochem Pharm Sci. 2023; 2(2): 65–69.
Debeturu SV, Tulandi, SS, Tiwow, GAR, Paat VI. Analgesic Activity Test of Ethanol Extract of Songgolangit Leaf (Tridax procumbens L.) Against White Rats (Rattus norvegicus). Biofar. Trop. 2022; 5(1): 66-72
Adeyemi DK, Ikwugbado RI, Adeyeye HM, Johnson OO. Investigation of the Phytochemicals, Metals Content and Antibacterial Activities of Commercial Herbal Preparations Sampled from Lagos Market, Nigeria: http://www.doi.org/10.26538/tjpps/v2i4.3. Trop J Phytochem Pharm Sci. 2023; 2(4): 105–113.
Rudiana T, Nurbayti S, Ashari TH, Zhorif SA, Suryani N. Comparison of Maceration and Soxhletation Methods on the Antioxidant Activity of the Bouea macrophylla Griff Plant. Jurnal Kimia Valensi. 2023; 9(2): 244-252.
Manurung H, Susanto D, Kusumawati E, Aryani R, Nugroho RA, Ratnakusuma, Rahmawati Z, Sari RD. Phytochemical, GC-MS analysisand antioxidant activities ofleaf methanolic extract of Lai (Durio kutejensis),the endemic plant of Kalimantan, Indonesia. Biodiversitas. 2022; 23(11): 5566-5573.
Riskianto, Windi W, Karnelasatri, Aruan M. Antioxidant Activity of 96% Ethanol Extract of Pepaya Jepang Leaves (Cnidoscolus aconitifolius (Mill.) I. M. Johnst) Using DPPH Method (1,1-diphenyl-2-picrylhydrazyl). Borneo J Pharm. 2022; 5(4): 315-324.
Kaushik D, Tanwar A, Davis J. Ethnopharmacological and phytochemical studies of Tridax procumbens Linn: a popular herb in ayurveda medicine. Int J of Eng Res and Tech (IJERT). 2020; 9(09): 758-768.
Yitayeh MM, Amanu MW. Chemical composition and antibacterial and antioxidant activities of stem bark essential oil and extract of solanecio gigas. Hindawi. 2022; 10.
Anuradha, G. & Mani, N. Bioactive compounds in ethanolic extract of Sansevieria roxburghiana leaves using GC-MS technique. Int J Bot Stud. 2021; 6(2): 01-04.
Salamone S, Appendino G, Khalili A, Pollastro F, Munoz E, Unciti-Broceta JD. Agathadiol, a labdane diterpenoid from juniper berries, is a positive allosteric modulator of CB1R. Fitoterapia. 2021; 155: 105059.
Xue W, Fu T, Zheng G, Tu G, Zhang Y, Yang F, Tao L, Yao L, Zhu F. Recent advances and challenges of the drugs acting on monoamine transporters. Curr Med Chem. 2020; 27(23): 3830-3876.
Habib A, Nargis A, Bi L, Zhao P, Wen L. Analysis of amphetaminic drug compounds in urine by headspace-dielectric barrier discharge ionization-mass spectrometry. Arab J Chem. 2020; 13(1): 2162-2170.
Hamed A, Mantawy E, El-Bakly W, Abdel-Mottaleb Y, and Azab S. Methyl palmitate: the naturally occurring cardioprotective agent. Arch Pharm Sci ASU. 2020; 4(1): 47-62.
Sari AI. Manajemen Reaksi Anafilaksis. Syntax Idea. 2023; 5(10): 1476-1490.
Gonzalez-Rivera ML, Barragan-Galvez JC, Gasca-Martínez D, Hidalgo-Figueroa S, Isiordia-Espinoza M, & Alonso-Castro AJ. In Vivo Neuropharmacological Effects of Neophytadiene. Molecules. 2023; 28(8): 3457.
Dutt K. Role of Antifungal Drugs in Combating Invasive Fungal Diseases. High Val Ferm Prod: Hum Health. 2019; 1: 103-144.
Altman KH, Bold CP, Gut M, Schurmann J, Agell DL, Diaz JF, Gertsch J. Syinthesis of mopholine-based analogs of (-)- zampanolide and their biological activity. Che Eur J. 2021; 27(9): 5936-5943.
Costantine FD, Robin IT, Mohamad AM, Bilal N, Hachem A, Rawan HC, Wassim NS. Laurus nobilis leaves extract protects against high fat diet-induced type 2 Diabetes in rats. J. Pharm Phyt. 2021; 13(3): 82-90.
Berg M, Polyzos KA, Agardh H, Baumgartner R, Forteza MJ, Kareinen I, Ketelhuth DF. 3-Hydroxyanthralinic acid metabolism controls the hepatic SREBP/lipoprotein axis, inhibits inflammasome activation in macrophages, and decreases atherosclerosis in Ldlr−/− mice. Cardio research. 2020; 116(12): 1948-1957.
Grafakou ME, Barda C, Skaltsa H. Secondary metabolites of Teucrium species with toxic effects. Teuc Spec: Bio and App. 2020; 211-230.
Zothanpuia, Passari AK, Leo VV, Chandra P, Kumar B, Nayak C, Singh BP. Bioprospection of actinobacteria derived from freshwater sediments for their potential to produce antimicrobial compounds. Micro cell fact. 2018; 17: 1-14.
Fedder D, Patel H, Saadabadi A. Atomoxetine. National Library of Medicine. 2018.
Wibawa IGKS, Suprapta DN, Khalimi K. Antagonistic Test of Endophytic Bacteria against Colletotrichum scovillei that Causes Anthracnose Disease in Large Chili (Capsicum annuum L.). J Agric Sci and Biotech. 2019; 8(1): 9.
Fresco-Cala, B, Batista AD, Cárdenas S. Molecularly imprinted polymer micro-and nano-particles: a review. Molecules. 2020; 25(20): 4740.
Lu Q, Liu T, Wang N, Dou Z, Wang K, Zuo Y. Nematicidal effect of methyl palmitate and methyl stearate against Meloidogyne incognita in bananas. J Agric Food Chem. 2020; 68(24): 6502-6510.
Earlia N, Rahmad R, Amin M, Prakoeswa CRS, Khairan K, Idroes R. The potential effect of fatty acids from Pliek U on epidermal fatty acid binding protein: Chromatography and bioinformatic studies. Sains Malay. 2019; 48(5): 1019-1024.
HSIAO YL, YEN JH. Enantioselective Effects of Imazapyr on Resistant Arabidopsis thaliana GH90. 臺灣農藥科學. 2021; 11: 63-79.
Julizan N. Validation of antioxidant activity determination by DPPH method. Kandaga-Media for Scientific Publication of Functional Position of Educational Personnel. 2019; 1(1).
Wibowo DP, Febriana Y, Riasari H, Auilifa DL. Essential oil composition, antioxidant and antibacterial activities of nutmeg (Myristica fragrans Houtt) from Garut West Java. Indo J Pharm Sci Tech. 2018; 5(3): 82-87.
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