Characterization of Physicochemical and Micromeritics Properties of Carboxymethylated Starches Derived from Vigna unguiculata Seeds and Pennisetum glaucum Grains with an Intermediate Degree of Substitution
http://www.doi.org/10.26538/tjpps/v1i1.5
Keywords:
starch, carboxymethylation, cowpea seeds, physicochemical, pearl millet grainsAbstract
Conventional commercial sources of starch for industrial applications are mainly based on staple foods, due to the worsening economic situation, there is a need to explore other underutilized unconventional sources. This study aims to extract, modify, and characterize starch from unconventional sources: cowpea (Vigna unguiculata) seeds and pearl millet (Pennisetum glaucum) grains. Starch was extracted from cowpea seeds and pearl millet grains by milling and precipitation in sodium hydroxide solution and water respectively. Carboxymethylation of the starch was carried out using monochloroacetic acid. The native starch, modified starches, and commercial brand-sodium starch glycolate were subjected to physicochemical and micromeritic characterization as well as spectroscopic and thermal analysis. The starch yields of 16.01 and 46.60% were obtained from cowpea seeds and pearl millet grains respectively. Starch samples with an intermediate degree of substitution (0.52-0.60) were obtained from both sources. The starch samples complied with British Pharmacopoeia specification and carboxymethylation generally resulted in improved properties (reduced gelatinization temperature, increased swelling capacity and flow properties). The pearl millet was made of oval-shaped granules with higher starch yield, reduced moisture content, and improved flow than the owpea starch, however, the latter contained cuboid-shaped granules, had higher densities, and reduced redispersion time. Carboxymethyl functional group was introduced, and the structure of starch was intact upon modification. Carboxymethyl starches obtained from cowpea seeds and pearl millet grains have desirable properties and can be potential sources of low-cost excipients for pharmaceutical formulations.
References
Apriyanto A, Compart J, Fettke J. A review of starch, a unique biopolymer – Structure, metabolism and in planta modifications. Plant Sci. 2022;1(318):111223.
Wani IA, Sogi DS, Hamdani AM, Gani A, Bhat NA, Shah A. Isolation, composition, and physicochemical properties of starch from legumes: A review. Starch - Stärke. 2016;68(9–10):834–845.
Santana ÁL, Angela M, Meireles A. New starches are the trend for industry applications: a review. Food and Public Health. 2014;4(5):229-241.
Azubuike CP, Adeluola AO, Mgboko MS, Madu SJ. Physicochemical and microbiological evaluation of acid modified native starch derived from Borassus aethiopum (Arecaceae) shoot. Trop J Pharm Res. 2018;17(5):883-890.
Ortiz R. Cowpeas from Nigeria: A Silent Food Revolution. Outlook Agric. 1998;27(2):125–128.
Saka JO, Agbeleye OA, Ayoola OT, Lawal BO, Adetumbi JA, Oloyede-Kamiyo QO. Assessment of varietal diversity and production systems of cowpea (Vigna unguiculata (L.) Walp.) in Southwest Nigeria. J Agric Rural Dev Trop Subtrop JARTS. 2018;119(2):43–52.
Ashogbon AO, Akintayo ET. Isolation and characterization of starches from two cowpea (Vigna unguiculata) cultivars. Int Food Res J. 2013;20(6):3093-3100.
Oyeyinka SA, Kayitesi E, Adebo OA, Oyedeji AB, Ogundele OM, Obilana AO, Njobeh PB Review on the physicochemical properties and potential food applications of cowpea (Vigna unguiculata) starch. Int J Food Sci Technol. 2021;56(1):52–60.
Giami SY. Compositional and nutritional properties of selected newly developed lines of cowpea (Vigna unguiculata L.Walp). J Food Compos Anal. 2005 Nov 1;18(7):665–673.
Punia Bangar S, Nehra M, Siroha AK, Petrů M, Ilyas RA, Devi U, Devi PD. Development and Characterization of Physical Modified Pearl Millet Starch-Based Films. Foods. 2021;10(7):1609.
Suma P F, Urooj A. Isolation and Characterization of Starch from Pearl Millet (Pennisetum typhoidium) Flours. International Journal of Food Properties. 2015;18(12):2675-2687.
Anirudhan TS, Parvathy J. Carboxymethyl starch as a polymeric plant-based excipient in drug delivery. Plant Sci Today. 2014;1(4):179–182.
Colussi R, Pinto VZ, El Halal SLM, Vanier NL, Villanova FA, e Silva RM, Zavareze E, Dias ARG. Structural, morphological, and physicochemical properties of acetylated high-, medium-, and low-amylose rice starches. Carbohydr Polym. 2014:103:405–413.
Ibikunle A, Sanyaolu N, Yussuf S, Ogunneye AL, Badejo OA, Olaniyi OM. Effects of chemical modification on functional and physical properties of African star apple kernel (Chrysophyllum albidnum) starch. Afr J Pure Appl Chem. 2019;13(1):1-11.
Madu SJ, Azubuike CP, Okubanjo O, Mohammed A, Emeje OM. Physicochemical and disintegrant properties of sodium Carboxymethyl starch derived from Borassus aethiopum(Arecaceae) shoot. J Polym Res. 2018 Jul 7;25(8):167.
Li SF, Mujyambere JM, Liu M. Synthesis of carboxymethyl starch with high degree of substitution by a modified dry process. In Advanced Materials Research 2011 (Vol. 233, pp. 306-310). Trans Tech Publications Ltd.
Ratnaningsih N, Suparmo, Harmayani E, Marsono Y. Composition, microstructure, and physicochemical properties of starches from Indonesian cowpea (Vigna unguiculata) varieties. Int Food Res J. 2016;23(5):2041–2049.
Yanli W, Wenyuan G, Xia L. Carboxymethyl Chinese yam starch: synthesis, characterization, and influence of reaction parameters. Carbohydr Res. 2009 Sep 8;344(13):1764–9.
Stojanović Ž, Jeremić K, Jovanović S. Synthesis of Carboxymethyl Starch. Starch - Stärke. 2000;52(11):413–419.
Oluwasina O, Wahab O, Umunna Q, Nwosa O. Dioscorea dumetorum Pax as an Alternative Starch Source for Industrial Applications. IOSR J Appl Chem. 2017;10(5):05–13.
Vashisht D, Pandey A, Jayaram Kumar K. Physicochemical and release properties of carboxymethylated starches of Dioscorea from Jharkhand. Int J Biol Macromol. 2015;74:523–529.
Basri SN, Zainuddin N, Hashim K, Yusof NA. Preparation and characterization of irradiated carboxymethyl sago starch-acid hydrogel and its application as metal scavenger in aqueous solution. Carbohydr Polym. 2016;138:34–40.
Mohapatra S, Siddiqui AA, Anwar M, Bhardwaj N, Akhter S, Ahmad FJ. Synthesis and characterization of novel carboxymethyl Assam Bora rice starch for the controlled release of cationic anticancer drug based on electrostatic interactions. AAPS PharmSciTech. 2018;19(1):134–47.
British Pharmacopoeia 2017 pdf Free Download [Internet]. Book Of Medical. [cited 2022 Aug 19]. Available from: https://www.booksofmedical.com/2022/05/britishpharmacopoeia-2017-pdf-free.html
Azubuike C, Fabiyi R, Oseni B, Igwilo C, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Lagos, College of Medicine Campus, PMB 12003, Surulere, Lagos, Nigeria. Evaluation of Disintegrant Potential of Carboxymethyl Starch Derived from Cyperus esculentus (Cyperaceae) Tubers. Trop J Nat Prod Res. 2019;3(7):246–251.
Adeyanju O, Olademehin OP, Hussaini Y, Nwanta UC, Adejoh AI, Plavec J. Synthesis and Characterization of Carboxymethyl Plectranthus esculentus Starch. A Potential Disintegrant. J Pharm Appl Chem. 2016;2(3):189–195.
Okunlola A, Akingbala O. Characterization and evaluation of acid-modified starch of Dioscorea oppositifolia (Chineseyam) as a binder in chloroquine phosphate tablets. Braz J Pharm Sci. 2013;49(4):699–708.
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