The Effects of 3,4-Methylenedioxymethamphetamine on Reproductive Hormones, Organ Weight, and Fertility Outcomes in Female Wistar Rats
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Abstract
Hormones play significant role in reproduction, and psychoactive substances such as 3,4-Methylenedioxymethamphetamine (MDMA) can significantly disrupt these hormonal activities. Substance abuse, once considered a male phenomenon, now records increasing women indulgence. MDMA's effects on female fertility remain understudied. This study assessed the impact of MDMA on body and organ weights, hormonal profile, and reproductive outcomes in female Wistar rats. Thirty female rats were divided into three groups (n = 30): Group A (control), Group B (80 mg/kg MDMA), and Group C (160 mg/kg MDMA). Hormonal assays and organ weight were evaluated. Data were analyzed using SPSS version 29.0, with significance at p ≤ 0.05. Body weight showed no significant differences (p = 0.21), but ovarian weight increased in treated groups. Uterine weight was unaffected. Group C showed significant body weight reduction (p = 0.01). LH levels declined significantly (p = 0.02); FSH and PRL remained unchanged. Group B had elevated progesterone, Group C showed increased estradiol, while testosterone decreased in Group B but increased in Group C. Pregnancy success was 100% in controls, 20% in Group B, and 0% in Group C. MDMA disrupted reproductive hormones and ovarian weight, leading to reduced fertility in female Wistar rats.
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References
United Nations Office on Drugs and Crime. World Drug Report 2019 [Internet]. Vienna: United Nations Publications; 2019. Available from: https://www.unodc.org/wdr2019
United Nations Office on Drugs and Crime. World Drug Report 2017 [Internet]. Vienna: United Nations Publications; 2017. Available from: https://www.unodc.org/wdr2017
World Health Organization. Global status report on alcohol and health 2018 [Internet]. Geneva: WHO; 2018. Available from: https://www.who.int/publications/i/item/9789241565639
Arpa S. Women and drugs: Drug use, incarcerated women and female drug dealers in Europe [Internet]. Lisbon: European Monitoring Centre for Drugs and Drug Addiction; 2017. Available from: https://www.emcdda.europa.eu
United Nations Office on Drugs and Crime. Drug use in Nigeria: 2018 National Drug Use Survey [Internet]. Vienna: United Nations Publications; 2018. Available from: https://www.unodc.org
Greenfield SF, Back SE, Lawson K, Brady KT. Substance abuse in women. Psychiatr Clin North Am. 2010;33(2):339–55. https://doi.org/10.1016/j.psc.2010.01.004
National Institute on Drug Abuse. Sex and gender differences in substance use [Internet]. 2020. Available from: https://nida.nih.gov/publications/research-reports/substance-use-in-women
Palamar JJ. MDMA use among U.S. high school seniors: Prevalence, correlates, and use-related harm. Prev Sci. 2016;17(3):347–55. https://doi.org/10.1007/s11121-015-0617-7
Meyer JS. 3,4-Methylenedioxymethamphetamine (MDMA): Current perspectives. Subst Abuse Rehabil. 2013;4:83–99. https://doi.org/10.2147/SAR.S37258
Stöhr T, Schramm M, Müller WE. Effects of MDMA on the rat reproductive system: Histopathological and biochemical findings. Toxicol Lett. 1998;102–103:231–7. https://doi.org/10.1016/S0378-4274(98)00232-1
Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983;54:275–87. https://doi.org/10.1007/BF01234480
National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the care and use of laboratory animals. 8th ed. Washington (DC): National Academies Press; 2011.
Chen L, Lu Y, Zhou YF, Wang Y, Zhan HF, Zhao YT, Wang YL, Zhang FF, Chen H, Li X. The effects of weight loss-related amenorrhea on women's health and the therapeutic approaches: a narrative review. Ann Transl Med. 2023;11(2):132. doi: 10.21037/atm-22-6366. PMID: 36819572; PMCID: PMC9929756.
Norris DO, Carr JA. Vertebrate endocrinology. 5th ed. Amsterdam: Academic Press; 2013.
Khalili M, Salehi I, Haghparast A. Reproductive hormonal alterations following MDMA (ecstasy) administration in male rats: involvement of hypothalamic GnRH. Neurosci Lett. 2017;653:268–73.
Sánchez V, Rodríguez M, Sabaté E, Esteban B, Camarasa J, Pubill D, et al. MDMA (Ecstasy) alters uterine contractility and increases oxidative stress. Reprod Toxicol. 2016;63:127–33.
Shahidi M, Motamedi F, Naghdi N, Ebrahimzadeh-Bideskan A, Oryan S. Sex-dependent effects of MDMA on anxiety and hormonal levels in adult rats. Iran J Basic Med Sci. 2018;21(7):678–84.
Zuloaga DG, Heck AL, De Guzman RM, Handa RJ. Roles for androgens in mediating sex differences in neuroendocrine stress responses. Biol Sex Differ. 2020;11(1):44.
Eferavware AS, Sakpa CL, Ezeuko VC. Oxidative effects of 3,4-methylenedioxymethamphetamine (MDMA) on the ovaries and uterus of adult female Wistar rats: implications for female reproductive health. Int J Forensic Med Invest. 2025;11(1).
Baumann MH, Wang X, Rothman RB. 3,4-Methylenedioxymethamphetamine (MDMA) neurotoxicity: evidence from preclinical studies. Neuropsychobiology. 2007;56(4):202–11.
Green AR, Mechan AO, Elliott JM, O'Shea E, Colado MI. The pharmacology and clinical pharmacology of 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy"). Pharmacol Rev. 2003;55(3):463–508.
McCann UD, Ricaurte GA. Adverse effects of MDMA on brain serotonin neurons: evidence from animal studies. Eur Neuropsychopharmacol. 1995;5(4):437–43.
Tsanas A, Berridge CW, Morrison AR. Maternal MDMA administration in mice leads to neonatal growth delay. Neurotoxicol Teratol. 2018;67:15–22.
García-Fernández A, Moreno AJ, Mate A. Effects of MDMA (ecstasy) and two of its metabolites on rat embryos in vitro. Toxicol Appl Pharmacol. 2017;328:26–34.