Histological Assessment and Antimicrobial Investigation of Pure Compound; 3,5,6,7- Tetrahydroxy-19-Vouacanoic Acid;(3β, 5α, 6β, 7β)-form,6,7-Dibenzoyl (Pulcherrimin A) Isolated from Caesalpinia pulcherrima Stem Bark http://www.doi.org/10.26538/tjpps/v2i1.1

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Osahon K. Ogbeide
Isaac U. Akhigbe
Charles A. Unuigbe
Osayemwenre Erharuyi
Vincent Imieje
Gabriel O. Benjamin
Kingsley Ikeke
Bosede Ayeni
Emmanuel Irabor
Joseph B. Owolabi
Abiodun Falodun


This study was aimed to investigate the histological effect and antimicrobial activity of a compound [3,5,6,7-tetrahydroxy-19-vouacanoic acid;(3β, 5α, 6β, 7β)-form,6,7-dibenzoyl] previously isolated from Caesalpinia pulcherrima stem bark prior to this study. Different doses (2, 4 and 8 mg/kg) of the compound was administered to Wistar rats (both sexes) for a 28-day period. After the 28-day administration, vital organs (liver, heart, kidney, lung, uterus and testes) were harvested from the animals to ascertain their organ weight variations and histology. Microbial activity of the compound was investigated (using zone of inhibition test and minimum inhibitory concentration) for the microorganisms (Bacillus subtilis, Klebsiella pneumoniae, P. aeruginosa, Staphylococcus aureus, Candida. albican and Trychophyton rubrum). The observed weight changes in organs for all treated groups in relative to control was not significant at p < 0.05 and no observed toxic abnormalities for all organs in treated groups upon their gross examination in relative to control. Highest zone inhibition was observed at 100 mg/kg for all tested microorganisms (B. subtilis, K. pneumonia, P. aeruginosa, S. aureus, C. albican and T. rubrum). with their corresponding MICs 11, 13, 16, 33, 30 and 31 mg/ml respectively. This study suggests
that the compound could be a potential candidate in view of developing new antimicrobial agent and potential novel drug as a vasodilator.


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Ogbeide, O. K., Akhigbe, I. U., Unuigbe, C. A., Erharuyi, O., Imieje, V., Benjamin, G. O., Ikeke, K., Ayeni, B., Irabor, E., Owolabi, J. B., & Falodun, A. (2023). Histological Assessment and Antimicrobial Investigation of Pure Compound; 3,5,6,7- Tetrahydroxy-19-Vouacanoic Acid;(3β, 5α, 6β, 7β)-form,6,7-Dibenzoyl (Pulcherrimin A) Isolated from Caesalpinia pulcherrima Stem Bark: http://www.doi.org/10.26538/tjpps/v2i1.1. Tropical Journal of Phytochemistry and Pharmaceutical Sciences, 2(1), 30–37. Retrieved from https://tjpps.org/home/article/view/15


Oladeji OS.The Characteristics and Roles of Medicinal Plants: Some important Medicinal Plants in Nigeria, Natural

Products: An Indian Journal 2016; 12.

Ayaz SA, Mujahid S, Shaikh A, Mukhtar M, Iftequar S. Antiulcerogenic Activity of Caesalpiniapulcherrima Leaves. Int. J Pharm Res & Allied Sci. 2015; 4:74-78.

Newman DJ. and Cragg GM. Natural products as source of new drugs over the last 25 years, J Nat Prod. 2007; 70:461- 77.

Hussein IH, M. Raad, R. Safa, R. Jurjus, A. Jurjus, Once Upon a Microscopic Slide: The Story of Histology. J CytolHistol 2015; 6:6.

Bennett HS. The role of histology in Medical Education and Biological Thinking. Anat Rec. 1956; 125:327-354.

Musumeci C Past, present and future: overview on histology & histopathology. J Histology & Histopathology 2014; 1(1):5. doi: 10.7243/2055-091X-1-5

Karaman I, Sahin F, Güllüce M, Ogütçü H, Sengül M, Adigüzel A. Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. J Ethnopharmacol 2003; 85: 231-5.

Falodun A, Imieje V, Erharuyi O, Ahomafor J, Langer P, Jacob M, Mohammed SK, Mark Hamann. Isolation of Antileishmanial, Antimalaria and Antimicrobial metabolites from Jatropha multifida. Asian Pac J Trop Biomed 2014; 4(5):374-378.

World Health organization, (WHO), (2018). Antimicrobial resistance. https://www.who.int/news-room/factsheets/detail/antimicrobial-resistance

Newman K. Herbs and spices: Their role in modern livestock production. In: Biotechnology in the Feed Industry (Eds. T.P.

Lyons and K.A. Jacques. Nottingham University Press, Loughborough, Leics, U.K 1997; 217-224.

Enegide C, David A, Fidelis SA, Dabum LJ. An Approach to Acute, Subacute, Subchronic and Chronic Toxicity Assessment in Animal Models. Toxicology International. 2015; 22:83-87.

, Anti-anaemic and Biosafety Examination of combined Telfairia occidentalis and Ipomoea batatas leaves extract, J Pharm Allied Sci. 2019; 16:3106-3113.

Randall RP. A Global Compendium of Weeds. Perth, Australia; Department of Agriculture and Food, Western Australia. 2012; 2:11224.

Pankaj N, Deepak N, Ranveer B A Review on Phytochemical and Pharmalogical aspects of Caesalpinia pulcherrima. Int. J. Res in Ayurveda and Pharmacy 2011; 2:416-421.

Patil AD, Freyer AJ, Webb RL, Zuber G, Reichwein R, Bean MF, Faucette L, Johnson RK. Pulcherrimins A-D, novel diterpene dibenzoates from Caesalpinia pulcherrima with selective activity against DNA repair-deficient yeast mutants. Tetrahedron 1997; 53;1583-1592.

Erharuyi O, Adhikari A, Falodun A, Imad R, Choudhary MI. Derivatization of cassane diterpenoids from Caesalpinia pulcherrima (L.) Sw. and evaluation of their cytotoxic and leishmanicidal activities. Tetrahedron Letters 2016; 57:2201-2206.

Patel SS, Verma NK, Chatterjee C, Gauthaman K. Screening of Caesalpinia pulcherrima Linn Flowers for Analgesic and Anti-inflammatory Activities. Int. J Applied Res Nat. 2010; Prod 3:1-5.

Pranithanchai W, Karalai C, Ponglimanon C, Subhadhirasakul S, Chantrapromma K Cassanediterpenoids from the stem of Caesalpinia pulcherrima, Phytochemistry. 2009; 70: 300-304.

Yodsaoue O, Karalai C, Ponglimanont C, Tewtrakul S, Chantrapromma S. Pulcherrins D–R, potential antiinflammatory diterpenoids from the roots of Caesalpinia pulcherrima. Tetrahedron 2011; 67:6838–6846.

Promsawan N, Kittakoop P, Boonphong S, Nongkunsarn P (2003) Antitubercular cassanefurano diterpenoids from the roots of Caesalpina pulcherrima. Planta Med. 2003; 69:776– 777.

Pawar CR, Mutha RE, Landg AD, Jadhav RB, Surana SJ Antioxidant and cytotoxic activities of Caesalpinia pulcherrima wood. Ind J Biochem Biophys. 2009; 46:198- 200.

Ogbeide OK, Dickson VO, Jebba BJ, Owhiroro DA, Olaoluwa MO, Imieje VCO, Erharuyi O, Owolabi BJ, Fasinu P, Falodun A. Anti-plasmodial and Acute Toxicity Studies of Fractions and Cassane-Type Diterpenoids from Stem Bark of Caesalpinia pulcherrima (L) Sw. Trop J Nat Prod Res. 2018; 2:179-184.

Ogbeide OK, Akhigbe IU, Unuigbe CA, Erharuyi O, Oseghale I, Imieje V, Iheanacho C, Ikeke K, Ayeni B, Irabor E, Owolabi JB, Falodun A. Isolation, characterization and in vivo anti-malarial investigation of pulcherrimin A from Caesalpinia pulcherrimastem bark, GSC Biol& Pharm Sci. 2020; 12:56-63.

Patil, A. D., Alan J. Freyer, R. Lee Webb, Gary Zuber, Rex Reichwein, Mark F. Bean, Leo Faucette and Randall K. Johnson. Pulcherrimins A-D, Novel Diterpene Dibenzoates from Caesalpinia pulcherrima with Selective Activity against DNA Repair-Deficient Yeast Mutants. Tetrahedron, 1997; 53(5):1583-1592.

Ogbeide OK, Akhigbe IU, Unuigbe CA, Erharuyi O, Imieje V, Benjamin GO, Ikeke K, Ayeni B, Irabor E, Owolabi JB, Falodun A. Haematological and Biochemical Examination of Pulcherrimin A isolated from Caesalpinia pulcherrim astem bark. Trop J Nat Prod Res. 2021; 5(11):2011-2015.

NIH Guide for the care and use of laboratory animals. U.S. Department of Health and Human Services, NIH Publication 1985; 86:1-83. 27. Cornel C, Aurelia NC, Manuella M, Simona N, Zbarcea EC, Nuta DC. Pharmacological Evaluation of Acute and Subacute Toxicity and Antidepressant effect after Acute Administration of Novel N-substituted Benzamides. FARMACIA 2010; 58:21-28.

Dina OA, Adedapo AA, Oyinloye O, Saba AB. Haematologocal effect of aqueous extract of Telfairia occidentalis. Afri J of Biomed Res. 2003; 3:181- 188.

Vinothkumar P, Sivaraj A, Ahmed KSZ, Sivamani P, Devi, K, Senthilkumar B (2010) Evaluation of antibacterial activities of Andrographis paniculata leaf extract against gram negative and gram-positive species by in vitro methods. J Pharmacol Res. 2010; 3:1513-1515.

National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility and MIC Tests; Approved Standards. 8th Edition. Information Supplement, Villanova. 2003; 23(1);M2-A8.

Lalitha MK, Manayani, DJ, Priya L, Jesudason MV, ThomasK, Steinhoff MC. Manual on antimicrobial susceptibility testing. Indian J Med Res. 2004; 106(4):100-103.

Dowe E, Ahonkhai I, Ayinde BA, Uwumarongie HO. Phytochemical and antimicrobial evaluation of the methanol stem extract and fraction of Massularia acuminata G. Don (Rubiaceae) against isolated odontopathogens. Ewemen J Microb Res. 2016; 2(1):13-21.

Bailey SA, Zidell RH, Pery RW. Relationship between organ weight and body/brain weight in the rat: what is the best analytical endpoint? Toxicologic Pathology 2004; 32:448- 466.

Carol SA. Acute, Subchronic and Chronic Toxicology. In: Derelanko M. J, Hollinger MA(Eds). CRC Handbook of Toxicology. CRC Press, Inc. FLA. 1985; 51-104.

Pramanik CK and Chatterje KT. Isolation, Characterisation and Sub-acute Toxicity of a New Compound PITC-2 Isolated from Tissue-Cultured Medicinal Plant, Pulchea indica (L.) less, J Biomed Pharmaceutical Sci. 2009; 3:50-54.

Wedro B, Anand BS, Stoppler MC Anatomy and Function of Liver. https://www.medicinenet.com/liver_anatomy_and_funtion/a

rticle. Assessed 25th January, 2022.

Zhou Z, Xu MJ, Gao B. Hepatocytes: a key cell type for innate immunity. Cellular Mol Immunology. 2016; 13:301- 315.

National Institute of Health (NIH). Your Kidneys & How They Work. https://www.niddk.nih.gov/healthinformation/kidny-disease/kidneys-how-they-work Assessed 25th January, 2022.

National Institute of Health (NIH) Anatomy of the Kidney and Ureter. https://training.seer.cancer.gov/kidney/anatomy/Assessed 25th January, 2022.

Gaea MM, and Tomislav M (2021) Structure & Function of the Heart. https://www.news-medical.net/health/Structureand-unction-of-the-Heart.aspx Assessed 25th January 2022; 1-5p

Hamirani YS and Ricciardi MJ Coronary Spasm Associated with Nitroglycerin Administration – A Case Report and Review of the Literature J Clinic Exp Cardiol. 2010; 1:103. doi: 10.4172/2155-9880.1000103

Aliya S. Effects of vasodilation and Arterial Resistance on Cardiac Output. J Clinic Exp Cardiol. 2011; 2:11. doi: 10.4172/2155-9880.1000170

Golneau S, Lacroix P, Soares-da-Silva P. Influence of Enalapril Therapy Schedule on the Progression of the Disease in Dilated Cardiomyopathic Syrian Hamsters (Bio TO-2 strain). J Clinic Experiment Cardiol. 2010; 1000-102.

Fancisco JLV, César LA, Alejandra RM, Isela RM, Miguel AZS. Vasodilator compounds Derived from Plants and Their Mechanisms of Action. Molecules 2013; 18:5814-5857.

A.L.S. America Lung Association, How Lung Works; The Respiratory System. https://www.lung.org/lung-healthdiseases/how-lungs-work. Assessed 25th February, 2021.

Brandt JP and Mandiga P (2021) Histology, Alveolar cells. https://www.ncbi.nim.nih.gov/books/NBK557542 Assessed 8th February, 2022.

Forfia PR, Vaidya A, Wiegers SE. Pulmonary heart disease: The heart-lung interaction nad its impact on patient Phenotypes. PulmCirc 2013; 3(1):5-19.

Willis-Gray MG, Young JC, Pate V, Funk MJ, J.M. Wu JM. Perioperative opioid prescriptions associated with stress incontinence and pelvic organ prolapse surgery. American J Obesterics Gynecology 2020; 223:894:e1-e9.

Gurevich R and Bilali L (2021) Fertility Challenges; Endometrium Conditions and Diseases. https://www.verywellfamily.com/understanding-theendometrium-1960066 Assessed 25th January, 2022.

Ibeh IN. and Uraih N. Practical Microbiology. 2003; 1:82- 93.

Feifei G, Weiping H, Shuzhen X, Su W, Xinxin L, Qian Z, Yuxing N, Lizhong H. Antimicrobial Resistance and Molecular Epidemiology of Staphylococcus aureus Causing Bloodstream Infections at Ruijin Hospital in Shanghai from 2013 to 2018. Scientific Reports. 2020; 10:6019. doi:10.1038/s41598-020-63248-5

Yang W, Chen X, Li Y, Guo S, Wang Z, Yu X (2020) Advances in Pharmacological Activities of Terpenoids. Natural Product Communications 2020; 15(3):1-13